Neurofilament light chain is a promising serum biomarker in spinocerebellar ataxia type 3

被引：67

作者：

Li, Quan-Fu ^{[1
,2
,3
]}

Dong, Yi ^{[1
,2
,3
]}

Yang, Lu ^{[1
,2
,3
]}

Xie, Juan-Juan ^{[1
,2
,3
]}

Ma, Yin ^{[1
,2
,3
]}

Du, Yi-Chu ^{[1
,2
,3
]}

Cheng, Hao-Ling ^{[4
,5
]}

Ni, Wang ^{[1
,2
,3
]}

Wu, Zhi-Ying ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Neurol, 88 Jiefang Rd, Hangzhou 310009, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Res Ctr Neurol, 88 Jiefang Rd, Hangzhou 310009, Zhejiang, Peoples R China

[3] Zhejiang Univ, Sch Med, Key Lab Med Neurobiol Zhejiang Prov, 88 Jiefang Rd, Hangzhou 310009, Zhejiang, Peoples R China

[4] Fujian Med Univ, Affiliated Hosp 1, Dept Neurol, Fuzhou, Fujian, Peoples R China

[5] Fujian Med Univ, Affiliated Hosp 1, Inst Neurol, Fuzhou, Fujian, Peoples R China

来源：

MOLECULAR NEURODEGENERATION | 2019年 / 14卷 / 01期

关键词：

Neurofilament light chain; Spinocerebellar ataxia type 3; Serum biomarker; DISEASE; BLOOD; PROTEIN;

D O I：

10.1186/s13024-019-0338-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Background Spinocerebellar ataxia type 3 (SCA3) is the most common subtype of autosomal dominantly inherited spinocerebellar ataxias (SCAs). No validated blood biomarker is available to assess either disease progression or therapeutic response. Neurofilament light chain (NfL) was recently proposed as a serum biomarker for many neurodegenerative disorders. The present study investigated whether NfL was a promising serum biomarker for SCA3. Methods Seventeen SCA3 patients and 9 controls were enrolled in cohort A, and 116 SCA3 individuals (preclinical and patients) and 91 controls were recruited as cohort B. We assessed whether serum NfL correlated with cerebrospinal fluid (CSF) NfL in cohort A and correlations between serum NfL levels and clinical features and brain volumes were determined in cohort B. The single-molecule array method was used to measure serum NfL levels. Disease severity was determined using the scale for the assessment and rating of ataxia (SARA) and the international cooperative ataxia rating scale (ICARS). Cerebellar and brainstem volumes were assessed using MRI neuroimaging measurements. Results Serum/CSF NfL levels in cohort A were elevated in SCA3 patients, and serum and CSF NfL exhibited a significant positive correlation (r = 0.9179, p < 0.0001). Levels of serum NfL in cohort B were significantly higher in preclinical SCA3 (15.03 +/- 7.49 vs 6.88 +/- 2.72 pg/ mL, p < 0.0001) and manifest SCA3 subjects (37.56 +/- 13.47 vs 9.07 +/- 6.02 pg/ mL, p < 0.0001) compared to those in controls. Serum NfL concentrations increased from early disease stage to the next stage. Levels of serum NfL in ATXN3 mutation carriers were positively associated with SARA (r = 0.5458, p < 0.0001) and ICARS scores (r = 0.5522, p < 0.0001). Significant negative associations with cerebellar volumes (r = - 0.4217, p = 0.0003) and brainstem volumes (r = - 0.4263, p = 0.0003) were observed. All changes remained significant after adjustment for age and CAG repeat. Conclusions Levels of serum NfL were significantly elevated in SCA3 individuals and correlated with disease severity. Serum NfL is a promising serum biomarker of disease onset and progression, and a potential candidate biomarker of treatment response in SCA3.

引用

页数：8

共 32 条

[31] Serum neurofilament light is increased in multiple system atrophy of cerebellar type and in repeat-expansion spinocerebellar ataxias: a pilot study [J].