DNA methylation status of MutS genes in ameloblastoma

被引：3
作者：
do Amaral-Silva, Gleyson Kleber [1 ]
Fontes Pereira, Thais dos Santos [2 ]
Rocha, Andre Caroli [3 ]
Linhares Almeida Mariz, Bruno Augusto [1 ]
Prado-Ribeiro, Ana Carolina [4 ]
Fonseca, Felipe Paiva [2 ]
Gomez, Ricardo Santiago [2 ]
Vargas, Pablo Agustin [1 ]
机构：
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, Brazil
[2] Univ Fed Minas Gerais, Sch Dent, Dept Oral Surg & Pathol, Belo Horizonte, MG, Brazil
[3] Univ Sao Paulo, Clin Hosp, Med Sch, Sao Paulo, Brazil
[4] ICESP FMUSP, Dent Oncol Serv, Inst Canc Estado Sao Paulo, Sao Paulo, Brazil
来源：
ORAL DISEASES | 2022年 / 28卷 / 07期
基金：
巴西圣保罗研究基金会;
关键词：
ameloblastoma; DNA methylation; DNA mismatch repair; quantitative real‐ time PCR;
D O I：
10.1111/odi.13887
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objectives Ameloblastoma is an odontogenic epithelial tumour with a low expression of mismatch repair system components. We aimed to investigate the methylation status of the genes MSH2, MSH3 and MSH6 (MutS group) in conventional ameloblastomas. Materials and Methods The ameloblastoma and dental follicle samples (n = 10 each) were collected from 20 different patients. Each ameloblastoma sample was sectioned into two fragments: one was paraffin-embedded while the other one, likewise the dental follicle samples, was fixed in RNAlater and frozen at -196 degrees C. All frozen samples were investigated for the MutS genes methylation levels, using the enzymatic restriction digestion and quantitative real-time PCR (qPCR) assay. The ameloblastoma paraffin-embedded samples were submitted to immunohistochemical reactions for MutS proteins detection and digitally quantification. Correlation analyses were performed between the immunohistochemical results and the respective gene methylation percentage. Results There are no significant differences between the MutS genes methylation levels in the ameloblastoma and the dental follicle. However, a strong negative correlation was found between MSH2 and MSH6 gene methylation status and their respective proteins expressions evaluated by immunohistochemistry. Conclusion Our results show that the genes methylations is in part responsible for decreasing the expression of MSH2 and MSH6 genes in ameloblastoma.
引用
收藏
页码：1901 / 1906
页数：6
相关论文
共 31 条
[1]   Allelic losses and DNA methylation at DNA mismatch repair loci in sporadic colorectal cancer [J].
Benachenhou, N ;
Guiral, S ;
Gorska-Flipot, I ;
Michalski, R ;
Labuda, D ;
Sinnett, D .
CARCINOGENESIS, 1998, 19 (11) :1925-1929
[2]  
BOLOGNAMOLINA R, 2018, MED ORAL PATOL ORAL, V23, pE12, DOI DOI 10.4317/MEDORAL.22210
[3]   Molecular pathology of Lynch syndrome [J].
Cerretelli, Guia ;
Ager, Ann ;
Arends, Mark J. ;
Frayling, Ian M. .
JOURNAL OF PATHOLOGY, 2020, 250 (05) :518-531
[4]   DNA methylation pattern of apoptosis-related genes in ameloblastoma [J].
Costa, S. F. S. ;
Pereira, N. B. ;
Pereira, K. M. A. ;
Campos, K. ;
de Castro, W. H. ;
Diniz, M. G. ;
Gomes, C. C. ;
Gomez, R. S. .
ORAL DISEASES, 2017, 23 (06) :779-783
[5]   Promoter hypermethylation of mismatch repair genes, hMLH1 and hMSH2 in oral squamous cell carcinoma [J].
Czerninski, R. ;
Krichevsky, S. ;
Ashhab, Y. ;
Gazit, D. ;
Patel, V. ;
Ben-Yehuda, D. .
ORAL DISEASES, 2009, 15 (03) :206-213
[6]   Prognostic significance of hMSH2, hMSH3, and hMSH6 expression in ameloblastoma [J].
do Amaral-Silva, Gleyson Kleber ;
Sanchez-Romero, Celeste ;
Wagner, Vivian Petersen ;
Martins, Manoela Domingues ;
Rebelo Pontes, Helder Antonio ;
Fregnani, Eduardo Rodrigues ;
Soares, Fernando Augusto ;
de Almeida, Oslei Paes ;
Rocha, Andre Caroli ;
Santos-Silva, Alan Roger ;
Fonseca, Felipe Paiva ;
Vargas, Pablo Agustin .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, 2017, 124 (03) :286-295
[7]   Epigenetic regulation of matrix metalloproteinase expression in ameloblastoma [J].
Farias, Lucyana Conceicao ;
Gomes, Carolina Cavalieri ;
Rodrigues, Marcela Carolina ;
de Castro, Wagner Henriques ;
Tanos Lacerda, Julio Cesar ;
Ferreira e Ferreira, Efigenia ;
Gomez, Ricardo Santiago .
BMC CLINICAL PATHOLOGY, 2012, 12
[8]   DNA methyltransferase expression in odontogenic cysts and tumours [J].
Gomes, Carolina Cavalieri ;
Ricieri Brito, Joao Artur ;
Andrade, Carlos Inacio ;
Gomez, Ricardo Santiago .
ONCOLOGY LETTERS, 2010, 1 (01) :143-146
[9]   DNA methyltransferase immunohistochemical expression in odontogenic tumours [J].
Guimaraes, Douglas Magno ;
Antunes, Daniella Moraes ;
Esteves Duarte, Carina Magalhaes ;
Ferro, Leonardo Borges ;
Nunes, Fabio Daumas .
JOURNAL OF ORAL PATHOLOGY & MEDICINE, 2015, 44 (01) :59-66
[10]   Early Dental Epithelial Transcription Factors Distinguish Ameloblastoma from Keratocystic Odontogenic Tumor [J].
Heikinheimo, K. ;
Kurppa, K. J. ;
Laiho, A. ;
Peltonen, S. ;
Berdal, A. ;
Bouattour, A. ;
Ruhin, B. ;
Caton, J. ;
Thesleff, I. ;
Leivo, I. ;
Morgan, P. R. .
JOURNAL OF DENTAL RESEARCH, 2015, 94 (01) :101-111
1234