Different signatures of miR-16, miR-30b and miR-93 in exosomes from breast cancer and DCIS patients

被引:62
作者
Ni, Qingtao [1 ]
Stevic, Ines [1 ]
Pan, Chi [1 ]
Mueller, Volkmar [2 ]
Oliviera-Ferrer, Leticia [2 ]
Pantel, Klaus [1 ]
Schwarzenbach, Heidi [1 ]
机构
[1] Univ Med Ctr Hamburg Eppendorf, Dept Tumor Biol, D-20246 Hamburg, Germany
[2] Univ Med Ctr Hamburg Eppendorf, Dept Gynecol, D-20246 Hamburg, Germany
来源
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词
CELL-FREE MICRORNAS; CLINICAL-RELEVANCE; SERUM-LEVELS; BIOGENESIS; BIOMARKERS; ONCOGENE; THERAPY; MIRNAS; GROWTH;
D O I
10.1038/s41598-018-31108-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Loading of microRNAs (miRNAs) into exosomes that are involved in cellular communication is a selective process. The current study investigates whether the enrichment of miRNAs in exosomes reflects the pathogenesis of breast cancer (BC) and ductal carcinoma in situ (DCIS). The levels of miRNAs were quantified in exosomes from plasma of 32 BC patients, 8 DCIS patients and 8 healthy women by TaqMan real-time PCR-based miRNA array cards containing 47 different miRNAs. Then, exosomal miR-16, miR-30b and miR-93 that displayed deregulation in the arrays were selected and analyzed in 111 BC patients, 42 DCIS patients and 39 healthy women by TaqMan real-time PCR. Identification of exosomes was performed by Western blot. The levels of exosomal miR-16 were higher in plasma of BC (p = 0.034) and DCIS (p = 0.047) patients than healthy women, and were associated with estrogen (p = 0.004) and progesterone (p = 0.008) receptor status. Particularly, in estrogen-positive patients miR-16 was significantly enriched in exosomes (p = 0.0001). Lower levels of exosomal miR-30b were associated with recurrence (p = 0.034). Exosomal miR-93 was upregulated in DCIS patients (p = 0.001). Our findings suggest that different signatures of miR-16, miR-30b and miR-93 in exosomes from BC and DCIS patients are associated with a particular biology of breast tumors.
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页数:10
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