Aqueous two-phase systems strategies for the recovery and characterization of biological products from plants

被引:45
作者
Aguilar, Oscar [1 ]
Rito-Palomares, Marco [1 ]
机构
[1] Ctr Biotecnol FEMSA, Dept Biotecnol & Ingn Alimentos, Monterrey 64849, NL, Mexico
关键词
aqueous two-phase systems; plant products; extraction; biological products; INSIDE-OUT VESICLES; PLASMA-MEMBRANES; COUNTERCURRENT DISTRIBUTION; EXCHANGE CHROMATOGRAPHY; RECOMBINANT PROTEINS; PHARMACEUTICAL PROTEINS; PENICILLIN ACYLASE; BETA-GLUCURONIDASE; PHOTOSYSTEM-II; PURIFICATION;
D O I
10.1002/jsfa.3956
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The increasing interest of the biopharmaceutical industry to exploit plants as economically viable production systems is demanding the development of new downstream strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological compounds. The present paper gives an overview of the efficient use of ATPS-based strategies for the isolation and partial purification of bioparticles from plant origin. Selected examples highlight the main advantages of this technique, i.e. scaling-up feasibility, process integration capability and biocompatibility. An overview of the recent approach of coupling ATPSs with traditional techniques to increase bioseparation process performance is discussed. A novel approach to characterization protein from plants combining ATPSs and two-dimensional electrophoresis (2-DE) is introduced as a tool for process development. In the particular case of products from plant origin, early success has demonstrated the potential application of ATPS-based strategies to address the major disadvantages of the traditional recovery and purification techniques. This literature review discloses the relevant contribution of ATPSs to facilitate the establishment of bioprocesses in the growing field of high-value products from plants. (C) 2010 Society of Chemical Industry
引用
收藏
页码:1385 / 1392
页数:8
相关论文
共 78 条
[1]   Plants as bioreactors:: A comparative study suggests that Medicago truncatula is a promising production system [J].
Abranches, R ;
Marcel, S ;
Arcalis, E ;
Altmann, F ;
Fevereiro, P ;
Stoger, E .
JOURNAL OF BIOTECHNOLOGY, 2005, 120 (01) :121-134
[2]   A peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root cultures:: inactivation by hydrogen peroxide and application in diagnostic kits [J].
Agostini, E ;
Hernández-Ruiz, J ;
Arnao, MB ;
Milrad, SR ;
Tigier, HA ;
Acosta, M .
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2002, 35 :1-7
[3]   Direct comparison between ion-exchange chromatography and aqueous two-phase processes for the partial purification of penicillin acylase produced by E. coli [J].
Aguilar, O ;
Albiter, V ;
Serrano-Carreón, L ;
Rito-Palomares, M .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 835 (1-2) :77-83
[4]  
AGUILAR O, 2007, THESIS ITESM MONTERR
[5]   Processing of soybean (Glycine max) extracts in aqueous two-phase systems as a first step for the potential recovery of recombinant proteins [J].
Aguilar, Oscar ;
Rito-Palomares, Marco .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (03) :286-293
[6]   Characterization of green-tissue protein extract from alfalfa (Medicago sativa) exploiting a 3-D technique [J].
Aguilar, Oscar ;
Glatz, Charles E. ;
Rito-Palomares, Marco .
JOURNAL OF SEPARATION SCIENCE, 2009, 32 (18) :3223-3231
[7]  
Åkerlund HE, 2000, METH BIOTEC, V11, P167
[8]   QUANTITATIVE SEPARATION OF SPINACH THYLAKOIDS INTO PHOTOSYSTEM-II-ENRICHED INSIDE-OUT VESICLES AND PHOTOSYSTEM-I-ENRICHED RIGHT-SIDE-OUT VESICLES [J].
AKERLUND, HE ;
ANDERSSON, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 725 (01) :34-40
[9]  
Åkerlund HE, 2000, METH BIOTEC, V11, P55
[10]   PHOTOSYNTHETIC ELECTRON-TRANSPORT FROM WATER TO NADP+ DRIVEN BY PHOTOSYSTEM-II IN INSIDE-OUT CHLOROPLAST VESICLES [J].
ALBERTSSON, PA ;
HSU, BD ;
TANG, GMS ;
ARNON, DI .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3971-3975