Evaluation of direct E-test on lower respiratory tract samples:: a rapid and accurate procedure for antimicrobial susceptibility testing

被引：23

作者：

Cercenado, Emilia ^{[1
]}

Cercenado, Sonia ^{[1
]}

Marin, Mercedes ^{[1
]}

Rico, Maria-Victoria ^{[1
]}

Vicente, Teresa ^{[1
]}

Bouza, Emilio ^{[1
]}

机构：

[1] Hosp Gen Univ Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas, Madrid 28007, Spain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 58卷 / 02期

关键词：

ventilator-associated pneumonia; E-test; rapid susceptibility testing; ICU; respiratory samples;

D O I：

10.1016/j.diagmicrobio.2006.12.021

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We compared the direct E-test susceptibility testing (DET) on respiratory samples with a standard microbroth dilution method (MBD) after quantitative cultures. A total of 152 samples from intensive care unit patients were processed by DET onto Mueller-Hinton agar. Oxacillin, piperacillin/tazobactam, cefepime, imipenem, ciprofloxacin, and amikacin were the antimicrobials evaluated. Cultures were 102 monomicrobials and 50 polymicrobials. Overall, 93.8% of the isolates were recovered by the DET. Among the 772 microorganism-antibiotic combinations evaluated, there was a total agreement with the MBD in 96.1%. There were 8 very major errors (1.03%), 15 major (1.94%), and 7 minor (0.91%). All discrepancies but one corresponded to polymicrobial cultures, and most occurred with cefepime (8 cases, 7.07%) and imipenem (7 cases, 6.18%). Readings of DET were easy to interpret and improved with transmitted light. DET on respiratory samples is a rapid technique that provides susceptibility results in 18 to 24 h comparable with these obtained by MBD. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：211 / 216

页数：6

共 30 条

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