Structure determination and analysis of a haemolytic gingipain adhesin domain from Porphyromonas gingivalis

被引:28
|
作者
Li, Nan [1 ]
Yun, Peter [3 ,4 ]
Nadkarni, Mangala A. [3 ,4 ]
Ghadikolaee, Nazila Babapoor [2 ,3 ,4 ]
Nguyen, Ky-Anh [2 ,3 ,4 ]
Lee, Mihwa [5 ]
Hunter, Neil [3 ,4 ]
Collyer, Charles A. [1 ]
机构
[1] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia
[2] Univ Sydney, Fac Dent, Sydney, NSW 2006, Australia
[3] Westmead Hosp, Inst Dent Res, Westmead Millennium Inst, Sydney, NSW, Australia
[4] Westmead Hosp, Ctr Oral Hlth, Sydney, NSW, Australia
[5] Univ Western Australia, Sch Biomed Biomol & Chem Sci, Nedlands, WA 6009, Australia
基金
澳大利亚研究理事会;
关键词
CYSTEINE PROTEINASES GINGIPAINS; CARBOHYDRATE-BINDING MODULES; HUMAN ERYTHROCYTES; MOLECULAR-CLONING; CRYSTAL-STRUCTURE; ENCODING GENE; ARG-GINGIPAIN; HEMOGLOBIN; COMPLEXES; KGP;
D O I
10.1111/j.1365-2958.2010.07123.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
P>Porphyromonas gingivalis is an obligately anaerobic bacterium recognized as an aetiological agent of adult periodontitis. P. gingivalis produces cysteine proteinases, the gingipains. The crystal structure of a domain within the haemagglutinin region of the lysine gingipain (Kgp) is reported here. The domain was named K2 as it is the second of three homologous structural modules in Kgp. The K2 domain structure is a 'jelly-roll' fold with two anti-parallel beta-sheets. This fold topology is shared with adhesive domains from functionally diverse receptors such as MAM domains, ephrin receptor ligand binding domains and a number of carbohydrate binding modules. Possible functions of K2 were investigated. K2 induced haemolysis of erythrocytes in a dose-dependent manner that was augmented by the blocking of anion transport. Further, cysteine-activated arginine gingipain RgpB, which degrades glycophorin A, sensitized erythrocytes to the haemolytic effect of K2. Cleaved K2, similar to that found in extracted Kgp, lacks the haemolytic activity indicating that autolysis of Kgp may be a staged process which is artificially enhanced by extraction of the protein. The data indicate a functional role for K2 in the integrated capacity conferred by Kgp to enable the porphyrin auxotroph P. gingivalis to capture essential haem from erythrocytes.
引用
收藏
页码:861 / 873
页数:13
相关论文
共 50 条
  • [1] Gingipain adhesin domains mediate Porphyromonas gingivalis adherence to epithelial cells
    Chen, T
    Duncan, MJ
    MICROBIAL PATHOGENESIS, 2004, 36 (04) : 205 - 209
  • [2] Humoral responses to Porphyromonas gingivalis gingipain adhesin domains in subjects with chronic periodontitis
    Nguyen, KA
    DeCarlo, AA
    Paramaesvaran, M
    Collyer, CA
    Langley, DB
    Hunter, N
    INFECTION AND IMMUNITY, 2004, 72 (03) : 1374 - 1382
  • [3] Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells
    Boisvert, Heike
    Duncan, Margaret J.
    CELLULAR MICROBIOLOGY, 2008, 10 (12) : 2538 - 2552
  • [4] THE LYSINE GINGIPAIN ADHESIN DOMAINS FROM PORPHYROMONAS GINGIVALIS INTERACT WITH ERYTHROCYTES AND ALBUMIN: STRUCTURES CORRELATE TO FUNCTION
    Ganuelas, L. A.
    Li, N.
    Yun, P.
    Hunter, N.
    Collyer, C. A.
    EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY, 2013, 3 (03): : 152 - 162
  • [5] Effectiveness of the B subunit of cholera toxin in potentiating immune responses to the recombinant hemagglutinin/adhesin domain of the gingipain Kgp from Porphyromonas gingivalis
    Zhang, P
    Yang, QB
    Balkovetz, DF
    Lewis, JP
    Clements, JD
    Michalek, SM
    Katz, J
    VACCINE, 2005, 23 (39) : 4734 - 4744
  • [6] Translocation of Porphyromonas gingivalis Gingipain Adhesin Peptide A44 to Host Mitochondria Prevents Apoptosis
    Boisvert, Heike
    Duncan, Margaret J.
    INFECTION AND IMMUNITY, 2010, 78 (08) : 3616 - 3624
  • [7] Molecular docking analysis of imidazole quinolines with gingipain R from Porphyromonas gingivalis
    Reddy, Kanumuru Rahul
    Rengasamy, Gayathri
    Sekaran, Surya
    Veeraraghavan, Vishnu Priya
    Sankaran, Kavitha
    Eswaramoorthy, Rajalakshmana
    BIOINFORMATION, 2023, 19 (01) : 88 - 93
  • [8] Analysis of the protease and adhesin domains of the PrpRI of Porphyromonas gingivalis
    Curtis, MA
    JOURNAL OF PERIODONTAL RESEARCH, 1997, 32 (01) : 133 - 139
  • [9] Vascular disruption by a 15kDa adhesin domain from Porphyromonas gingivalis.
    Paramaesvaran, M
    Layman, D
    Harty, D
    Relf, W
    Rathsam, C
    Hunter, N
    JOURNAL OF DENTAL RESEARCH, 1998, 77 : 644 - 644
  • [10] The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis
    Li, Nan
    Yun, Peter
    Jeffries, Cy M.
    Langley, David
    Gamsjaeger, Roland
    Church, W. Bret
    Hunter, Neil
    Collyer, Charles A.
    MOLECULAR MICROBIOLOGY, 2011, 81 (05) : 1358 - 1373