Corynebacterium defluvii sp nov., isolated from Sewage

A Gram-positive, aerobic, non-motile, rod-shapeds, catalase-positive, and oxidase-negative strain, designated Y49(T), was isolated from sewage collected from Jilin Agricultural University, China. It grew at 20-40 degrees C (optimum at 30 degrees C), at pH 6.0-8.0 (optimum at 7.0) and at 0-1.0% sodium chloride (optimum at 0%). The major isoprenoid quinone was menaquinone-8 (MK-8) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, four unidentified lipids, and two unidentified aminolipids. The peptidoglycan was meso-diaminopimelic acid. The cell-wall sugars were galactose, arabinose, and glucose. The fatty acids were C-9:0, C-16:0, C-16:1 omega 9c, C-17:1 omega 9c, C-18:3 omega 6c (6,9,12), C-18:1 omega 9c, and C-18:0. The DNA G+C content was 51.4 mol%. Based on the 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain Y49(T) were Corynebacterium efficiens DSM 44549(T) (97.5%), Corynebacterium callunae DSM 20147(T) (97.2%), Corynebacterium deserti GIMN 1.010(T) (96.8%), Corynebacterium glutamicum ATCC 13032(T) (96.4%), and other species belonging to this genus (92.3-95.4%). The DNA-DNA relatedness value between strain Y49(T) and C. efficiens DSM 44549(T), C. callunae DSM 20147(T), C. deserti GIMN1.010(T), and C. glutamicum ATCC 13032(T) was 25.5 +/- 2.0%, 21.1 +/- 1.0%, 16.5 +/- 0.5%, and 13.5 +/- 0.9%, respectively. Based on the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain Y49(T) represents a novel species of the genus Corynebacterium, for which the name Corynebacterium defluvii sp nov. is proposed. The type strain is Y49(T) (=KCTC 39731(T) =CGMCC 1.15506(T)).