Ultra-high performance liquid chromatographic and mass spectrometric analysis of active vitamin B12 in cells of Propionibacterium and fermented cereal matrices

被引：68

作者：

Chamlagain, Bhawani ^{[1
]}

Edelmann, Minnamari ^{[1
]}

Kariluoto, Susanna ^{[1
]}

Ollilainen, Velimatti ^{[1
]}

Piironen, Vieno ^{[1
]}

机构：

[1] Univ Helsinki, Dept Food & Environm Sci, FI-00014 Helsinki, Finland

来源：

FOOD CHEMISTRY | 2015年 / 166卷

基金：

芬兰科学院;

关键词：

Vitamin B12; Propionibacterium freudenreichii; Fermented cereal matrix; UHPLC; UPLC; Immunoaffinity purification; IMMUNOAFFINITY EXTRACTION; MICROBIOLOGICAL ASSAY; DAIRY-PRODUCTS; FOOD-PRODUCTS; FREUDENREICHII; VALIDATION; CLEANUP; FOLATE; MILK; HPLC;

D O I：

10.1016/j.foodchem.2014.06.068

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive and selective method is needed to analyse in situ produced vitamin B12 in plant-based materials, potential new dietary sources of vitamin B12. A UHPLC/UV method was developed and validated for the determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matrices fermented by P. freudenreichii. An Acquity HSS T3 C18 column resulted in a baseline separation, a calibration curve of excellent linearity and a low limit of detection (0.075 ng/5 mu L injection). As confirmed by UHPLC MS, the active vitamin B12 could be separated from pseudovitamin B12. The recovery of vitamin B12 from purified spiked cereal matrices was good (>90%; RSD < 5%). A nutritionally relevant amount of active vitamin B12 was produced by P. freudenreichii in cereal malt matrices (up to 1.9 mu g/100 g) in 24 h at 28 degrees C. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：630 / 638

页数：9

共 30 条

[1]

[Anonymous], 2006, 98623 AOAC INT

[2]

Ball G.F. M., 2006, Vitamins in foods analysis, bioavailability, and stability, V156

[3]

Campos-Giménez E, 2008, J AOAC INT, V91, P786

[4] Human vitamin B12 absorption measurement by accelerator mass spectrometry using specifically labeled 14C-cobalamin [J].

Carkeet, C ;

Dueker, SR ;

Lango, J ;

Buchholz, BA ;

Miller, JW ;

Green, R ;

Hammock, BD ;

Roth, JR ;

Anderson, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (15) :5694-5699

[5] A LC/UV/Vis method for determination of cyanocobalamin (VB12) in multivitamin dietary supplements with on-line sample clean-up [J].

Chen, Pei ;

Wolf, Wayne R. ;

Castanheira, Isabel ;

Sanches-Silva, Ana .

ANALYTICAL METHODS, 2010, 2 (08) :1171-1175

[6] Determination of Vitamin B12 in meat products by RP-HPLC after enrichment and purification on an immunoaffinity column [J].

Guggisberg, D. ;

Risse, M. C. ;

Hadorn, R. .

MEAT SCIENCE, 2012, 90 (02) :279-283

[7] VITAMIN-B-12 - PLANT SOURCES, REQUIREMENTS, AND ASSAY [J].

HERBERT, V .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1988, 48 (03) :852-858

[8] Determination of vitamin B12 in food products and in premixes by reversed-phase high performance liquid chromatography and immunoaffinity extraction [J].

Heudi, O ;

Kilinç, T ;

Fontannaz, P ;

Marley, E .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1101 (1-2) :63-68

[9] Screening of a natural biodiversity of lactic and propionic acid bacteria for folate and vitamin B12 production in supplemented whey permeate [J].

Hugenschmidt, Selina ;

Schwenninger, Susanne Miescher ;

Gnehm, Nicole ;

Lacroix, Christophe .

INTERNATIONAL DAIRY JOURNAL, 2010, 20 (12) :852-857

[10]

Indyk HE, 2002, J AOAC INT, V85, P72

← 1 2 3 →