Echinacea species and alkamides inhibit prostaglandin E2 production in RAW264.7 mouse macrophage cells

Inhibition of prostaglandin E-2 (PGE(2)) production in lipopolysaccharicle-stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunciassay following treatments with Echinacea extracts or synthesized alkamides. Results indicated that ethanol extracts diluted in media to a concentration of 15 mu g/mL from E angustifolia, E pallida, E. simulata, and E sanguinea significantly inhibited PGE2 production. In further studies, PGE2 production was significantly reduced by all synthesized alkamides assayed at 50 mu M, by Bauer alkamides 8, 12A analogue, and 14, Chen alkamide 2, and Chen alkamide 2 analogue at 25 mu M and by Bauer alkamide 14 at 10 mu M. Cytotoxicity did not play a role in the noted reduction of PGE2 production in either the Echinacea extracts or synthesized alkamides. High-performance liquid chromatography analysis identified individual alkamides present at concentrations below 2.8 mu M in the extracts from the six Echinacea species (15 mu g/mL crude extract). Because active extracts contained <2.8 mu M of specific alkamide and the results showed that synthetic alkamides must have a minimum concentration of 10 mu M to inhibit PGE2, it is likely that alkamides may contribute toward the anti-inflammatory activity of Echinacea in a synergistic or additive manner.