Comparison of saponin composition and content in wild soybean (Glycine soja Sieb. and Zucc.) before and after germination

Eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by LC-PDA/MS/MS. Saponin composition and content were varied according to tissues and accessions. The average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 +/- 3.13 mu mol. In two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 +/- 6.52 mu mol. Group A saponins were highly concentrated in seed hypocotyl (4.04 +/- 0.71 mu mol). High concentration of DDMP saponins (7.37 +/- 5.22 mu mol) and Sg-6 saponins (2.19 +/- 0.59 mu mol) was found in cotyledonary leaf. In seedlings, the amounts of group A and Sg-6 saponins reduced 2.3- and 1.3-folds, respectively, while DDMP + B + E saponins increased 2.5-fold than those of mature seeds. Our findings show that the group A and Sg-6 saponins in mature seeds were degraded and/or translocated by germination whereas DDMP saponins were newly synthesized.