Niche partitioning facilitates coexistence of closely related honey bee gut bacteria

被引:76
作者
Brochet, Silvia [1 ]
Quinn, Andrew [1 ]
At Mars, Ruben [2 ]
Neuschwander, Nicolas [1 ]
Sauer, Uwe [2 ]
Engel, Philipp [1 ]
机构
[1] Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland
[2] Swiss Fed Inst Technol, Inst Mol Syst Biol, Zurich, Switzerland
基金
瑞士国家科学基金会; 欧洲研究理事会;
关键词
MICROBIOTA; EVOLUTION; DIVERSITY; HOST; COOPERATION; COMPETITION; ECOLOGY;
D O I
10.7554/eLife.68583
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic bees and in liquid cultures in the presence of either pollen (bee diet) or simple sugars. Although the four species engaged in negative interactions, they were able to stably coexist, both in vivo and in vitro. However, coexistence was only possible in the presence of pollen, and not in simple sugars, independent of the environment. Using metatranscriptomics and metabolomics, we found that the four species utilize different pollen-derived carbohydrate substrates indicating resource partitioning as the basis of coexistence. Our results show that despite longstanding host association, gut bacterial interactions can be recapitulated in vitro providing insights about bacterial coexistence when combined with in vivo experiments.
引用
收藏
页数:23
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