High-throughput protein crystallization

被引：71

作者：

Hui, R

Edwards, A

机构：

[1] Affinium Pharmaceut, Toronto, ON, Canada

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5S 1A1, Canada

[3] Univ Toronto, Hlth Network, Ontario Ctr Struct Proteom, Toronto, ON, Canada

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2003年 / 142卷 / 01期

关键词：

D O I：

10.1016/S1047-8477(03)00046-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput structural biology is a focus of a number of academic and pharmaceutical laboratories around the world. The use of X-ray crystallography in these efforts is critically dependent on high-throughput protein crystallization. The application of current protocols yields crystal leads for approximately 30% of the input proteins and well-diffracting crystals for a smaller fraction. Increasing the success rate will require a multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation. (C) 2003 Published by Elsevier Science (USA).

引用

页码：154 / 161

页数：8

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