Synthetic surfaces for human embryonic stem cell culture

被引：57

作者：

Kolhar, Poornima ^{[1
,2
]}

Kotamraju, Venkata Ramana ^{[1
]}

Hikita, Sherry T. ^{[2
]}

Clegg, Dennis O. ^{[2
]}

Ruoslahti, Erkki ^{[1
,2
]}

机构：

[1] Univ Calif Santa Barbara, Sanford Burnham Med Res Inst, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Ctr Stem Cell Biol & Engn, Neurosci Res Inst, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF BIOTECHNOLOGY | 2010年 / 146卷 / 03期

关键词：

Human embryonic stem cells; Extracellular matrix; RGD peptide; Biomaterials; FEEDER-LAYER-FREE; SERUM-FREE; GROWTH;

D O I：

10.1016/j.jbiotec.2010.01.016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human embryonic stem cells (hESCs) have numerous potential biomedical applications owing to their unique abilities for self-renewal and pluripotency. Successful clinical application of hESCs and derivatives necessitates the culture of these cells in a fully defined environment. We have developed a novel peptide-based surface that uses a high-affinity cyclic RGD peptide for culture of hESCs under chemically defined conditions. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：143 / 146

页数：4

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