Electrical impedance spectroscopy of gel embedded neuronal cells based on a novel impedimetric biosensor

被引：1

作者：

Jacobs, T. ^{[1
]}

Valero, T. ^{[2
]}

Naumann, M. ^{[3
]}

Kintzios, S. ^{[2
]}

Hauptmann, P. ^{[1
]}

机构：

[1] Otto von Guericke Univ, Inst Micro & Sensor Syst, D-39106 Magdeburg, Germany

[2] Agr Univ Athens, Fac Agr Biotechnol, GR-11855 Athens, Greece

[3] Otto Von Guericke Univ, Inst Expt Internal Med, D-39120 Magdeburg, Germany

来源：

PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE | 2009年 / 1卷 / 01期

关键词：

Impedimetric biosensor; Gel embedded neuronal cells; Electrical impedance spectroscopy; On-line monitoring;

D O I：

10.1016/j.proche.2009.07.065

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this contribution we focus on the on-line analysis of neuronal cells embedded in gel matrices by means of variations in their dielectric and conductive properties. For impedance measurement of thin gel films in flow-through regime a novel impedimetric biosensor was developed. A technique for the preparation of gels containing Neuro-2a neuroblastoma cells (N2a) in between the measurement electrodes was set up. Impedance spectra of gels with N2a cells were analyzed and cell viability was tested. Experimental results showed that even at frequencies (>>1 MHz) and a gel concentration of 2 % with similar to 8300 cells/mu L conductive properties dominate the spectrum.

引用

页码：261 / +

页数：2

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