Comparison of Groundnut bud necrosis virus isolates based on movement protein (NSm) gene sequences

被引:15
作者
Akram, M
Jain, RK [1 ]
Chaudhary, V
Ahlawat, YS
Khurana, SMP
机构
[1] Indian Agr Res Inst, Div Plant Pathol, Unit Plant Virol, New Delhi 110012, India
[2] Cent Potato Res Inst, Shimla 171001, Himachal Prades, India
关键词
Groundnut bud necrosis virus; NSm gene; tospovirus;
D O I
10.1111/j.1744-7348.2004.tb00385.x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The nucleotide and amino acid sequences of the movement protein (NSm) genes of five isolates of Groundnut bud necrosis virus (GBNV) originating from different hosts and parts of India such as cowpea and tomato from Kerala, groundnut from Tamil Nadu, and potato from Madhya Pradesh and Rajasthan were determined and compared to the known NSm sequences. Sequence analysis revealed that the NSm genes of GBNV isolates were identical in length (924 bp encoding 307 amino acids). GBNV isolates shared maximum identity (98-100%) at amino acid levels with GBNV-Type isolate, while 82-83% and 34-65% amino acid sequence identities were observed with Watermelon silver mottle virus and other Tospoviruses respectively. The NSm genes among GBNV isolates originating from different hosts and locations appeared highly conserved (93-100%), suggesting their common origin.
引用
收藏
页码:285 / 289
页数:5
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