Regional variations in stiffness in live mouse brain tissue determined by depth-controlled indentation mapping

被引：71

作者：

Antonovaite, Nelda ^{[1
,2
]}

Beekmans, Steven V. ^{[1
,2
]}

Hol, Elly M. ^{[3
,4
,5
]}

Wadman, Wytse J. ^{[5
]}

Iannuzzi, Davide ^{[1
,2
]}

机构：

[1] Vrije Univ Amsterdam, Dept Phys & Astron, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, LaserLab Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands

[3] Univ Med Ctr Utrecht, Dept Translat Neurosci, Brain Ctr Rudolf Magnus, NL-3584 CG Utrecht, Netherlands

[4] Netherlands Inst Neurosci, NL-1105 BA Amsterdam, Netherlands

[5] Univ Amsterdam, Swammerdam Inst Life Sci, Ctr Neurosci, NL-1098 XH Amsterdam, Netherlands

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

欧洲研究理事会;

关键词：

MECHANICAL-PROPERTIES; NERVOUS-SYSTEM; WHITE-MATTER; RAT-BRAIN; IN-VITRO; MODELS; GROWTH; MATURATION; BEHAVIOR; TENSION;

D O I：

10.1038/s41598-018-31035-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mechanical properties of brain tissue play a pivotal role in neurodevelopment and neurological disorders. Yet, at present, there is no consensus on how the different structural parts of the tissue contribute to its stiffness variations. Here, we have gathered depth-controlled indentation viscoelasticity maps of the hippocampus of acute horizontal live mouse brain slices. Our results confirm the highly viscoelestic nature of brain tissue. We further show that the mechanical properties are non-uniform and at least related to differences in morphological composition. Interestingly, areas with higher nuclear density appear to be softer than areas with lower nuclear density.

引用

页数：11

共 69 条

[1] Extracellular Matrix: Functions in the Nervous System [J].

Barros, Claudia S. ;

Franco, Santos J. ;

Mueller, Ulrich .

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2011, 3 (01) :1-24

[2] Characterizing tissue stiffness at the tip of a rigid needle using an opto-mechanical force sensor [J].

Beekmans, S. V. ;

Iannuzzi, D. .

BIOMEDICAL MICRODEVICES, 2016, 18 (01) :1-8

[3] A metrological approach for the calibration of force transducers with interferometric readout [J].

Beekmans, S. V. ;

Iannuzzi, D. .

SURFACE TOPOGRAPHY-METROLOGY AND PROPERTIES, 2015, 3 (02)

[4] Minimally Invasive Micro-Indentation: mapping tissue mechanics at the tip of an 18G needle [J].

Beekmans, Steven V. ;

Emanuel, Kaj S. ;

Smit, Theodoor H. ;

Iannuzzi, Davide .

SCIENTIFIC REPORTS, 2017, 7

[5] Microglia mechanics: immune activation alters traction forces and durotaxis [J].

Bollmann, Lars ;

Koser, David E. ;

Shahapure, Rajesh ;

Gautier, Helene O. B. ;

Holzapfel, Gerhard A. ;

Scarcelli, Giuliano ;

Gather, Malte C. ;

Ulbricht, Elke ;

Franze, Kristian .

FRONTIERS IN CELLULAR NEUROSCIENCE, 2015, 9 :1-16

[6] Brain Extracellular Matrix in Neurodegeneration [J].

Bonneh-Barkay, Dafna ;

Wiley, Clayton A. .

BRAIN PATHOLOGY, 2009, 19 (04) :573-585

[7]

Brands D W, 2000, Stapp Car Crash J, V44, P249

[8] Mechanical characterization of human brain tissue [J].

Budday, S. ;

Sommer, G. ;

Birkl, C. ;

Langkammer, C. ;

Haybaeck, J. ;

Kohnert, J. ;

Bauer, M. ;

Paulsen, F. ;

Steinmann, P. ;

Kuhl, E. ;

Holzapfel, G. A. .

ACTA BIOMATERIALIA, 2017, 48 :319-340

[9] Mechanical properties of gray and white matter brain tissue by indentation [J].

Budday, Silvia ;

Nay, Richard ;

de Rooij, Rijk ;

Steinmann, Paul ;

Wyrobek, Thomas ;

Ovaert, Timothy C. ;

Kuhl, Ellen .

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2015, 46 :318-330

[10] Towards child versus adult brain mechanical properties [J].

Chatelin, S. ;

Vappou, J. ;

Roth, S. ;

Raul, J. S. ;

Willinger, R. .

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2012, 6 :166-173

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