Analyzing autophagy in zebrafish

被引：42

作者：

He, Congcong

Klionsky, Daniel J. ^{[1
]}

机构：

[1] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA

来源：

AUTOPHAGY | 2010年 / 6卷 / 05期

关键词：

lysosome; protein degradation; protein targeting; stress; vacuole;

D O I：

10.4161/auto.6.5.12092

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism. 1 This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.

引用

收藏

页码：642 / 644

页数：3

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