Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213

被引：20

作者：

Li, Jinlong ^{[1
,2
]}

Shen, Weijia ^{[1
,2
]}

Fan, Guangsen ^{[1
,3
]}

Li, Xiuting ^{[1
,2
]}

机构：

[1] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Fucheng Rd, Beijing 100048, Peoples R China

[2] Beijing Higher Inst, Engn Res Ctr Food Addit & Ingredients, Beijing 100048, Peoples R China

[3] Beijing Key Lab Flavor Chem, Beijing 100048, Peoples R China

来源：

3 BIOTECH | 2018年 / 8卷 / 09期

基金：

中国国家自然科学基金;

关键词：

B; pyrrocinia; Lipase; Screening; Purification; Characterization; SOLVENT-TOLERANT LIPASE; MICROBIAL LIPASES; INDUSTRIAL APPLICATIONS; CANDIDA-ANTARCTICA; BACTERIAL LIPASES; FUNGAL LIPASES; TRANSESTERIFICATION; BIOTECHNOLOGY;

D O I：

10.1007/s13205-018-1414-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of this lipase was pH 8.0 and 50 degrees C, respectively. The lipase retained > 85% activity in isopropanol and acetone at 30 degrees C for 10 mM but the activity was reduced to 10.6% in n-hexane. Mg2+, Al3+, Mn2+, and Fe3+ enhanced lipase activity at both 1 mM and 5 mM concentrations. p-NPP, a long-chain acyl group 4-NP ester, appeared to be a good substrate candidate.

引用

页数：12

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