Qualitative and Quantitative Analyses of Active Constituents in Trollius ledebourii

被引:14
作者
Liao, Man [1 ]
Cheng, Xiaoye [1 ]
Zhang, Xia [1 ]
Diao, Xinpeng [1 ]
Liang, Caijuan [1 ]
Zhang, Lantong [1 ]
机构
[1] Hebei Med Univ, Sch Pharm, Dept Pharmaceut Anal, 361 East Zhongshan Rd, Shijiazhuang 050017, Hebei, Peoples R China
关键词
PERFORMANCE LIQUID-CHROMATOGRAPHY; FLAVONE C-GLYCOSIDES; MASS-SPECTROMETRY; TOF-MS; TROLLFLOWERS; CHINENSIS; FLOWERS; EXTRACT;
D O I
10.1093/chromsci/bmy035
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Trollius ledebourii has been more involved in Mongolianmedicine and is often used as a type of tea for heat-clearing and detoxifying in the populus. In this study, a rapid and sensitive method was established for the qualitative and quantitative analyses of the major constituents in T. ledebourii. Ultra-highperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was developed for the identification of themulti-constituents in T. ledebourii. A total of 37 chemical constituents in T. ledebourii extract were unambiguously or tentatively identified, including 17 flavonoid glycosides, 6 flavones, 3 flavonols, 1 dihydroflavone, 8 phenolic acids, 1 amide and 1 triterpene. Pectolinarin, naringenin, isorhamnetin, diosmetin, protocatechuic acid, paeonol, caffeic acid and ferulic acid were first detected in T. ledebourii and the buttercup family. High-performance liquid chromatography-quadrupole ion trap tandemmass spectrometry was applied for the simultaneous determination of 11 compounds, which were either with high contents or strong bioactivities. Satisfactory linearity was achieved with a wide linear range and fine determination coefficient (r > 0.9987). The overall recoveries ranged from 98.07 to 101.2%, and the precision in terms of RSD was < 0.74%. The results might provide the basis for quality control analysis of T. ledebourii.
引用
收藏
页码:619 / 635
页数:17
相关论文
共 36 条
[1]  
[Anonymous], 2015, PHARM COMM PEOPL REP, P1080
[2]  
Bai Y. H., 2010, CLIN J TRADITIONAL C, V22, P386
[3]   Antiviral flavonoid-type C-glycosides from the flowers of Trollius chinensis [J].
Cai, SQ ;
Wang, RF ;
Yang, XW ;
Shang, MY ;
Ma, CM ;
Shoyama, Y .
CHEMISTRY & BIODIVERSITY, 2006, 3 (03) :343-348
[4]   Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry [J].
Guo, Panpan ;
Yan, Wenying ;
Han, Qingjie ;
Wang, Chunying ;
Zhang, Zijian .
JOURNAL OF SEPARATION SCIENCE, 2015, 38 (07) :1156-1163
[5]  
Hu X. G., 2012, FAMILY MED, V6, P54
[6]  
[黄睿 Huang Rui], 2012, [中草药, Chinese Traditional and Herbal Drugs], V43, P670
[7]  
Li GuiLan Li GuiLan, 2016, China Journal of Traditional Chinese Medicine and Pharmacy, V31, P275
[8]  
Li L., 2007, THESIS, P1
[9]   Determination and pharmacokinetics of orientin in rabbit plasma by liquid chromatography after intravenous administration of orientin and Trollius chinensis Bunge extract [J].
Li, Xiaoqin ;
Huo, Taoguang ;
Qin, Feng ;
Lu, Xiumei ;
Li, Famei .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 853 (1-2) :221-226
[10]  
Li Y. R., 2012, HERALD MED, V31, P1500