Special application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiological diagnostics

被引：6

作者：

Nagy Erzsebet ^{[1
]}

Abrok Marianna ^{[1
]}

Bartha Noem ^{[1
]}

Bereczki Laszlo ^{[1
]}

Juhasz Emese ^{[2
]}

Kardos Gabor ^{[3
]}

Kristof Katalin ^{[2
]}

Miszti Cecilia ^{[3
]}

Urban Edit ^{[1
]}

机构：

[1] Klin Mikrobiol Diagnosztikai Intezet, Altalanos Orvostudomanyi Kar, Szegedi Tud Egyet, Szeged, Hungary

[2] Semmelweis Egyet, Altalanos Orvostudomanyi Kar, Lab Med Intezet, Klin Mikrobiol Diagnosztikai Lab, Budapest, Hungary

[3] Debreceni Egyet, Altalanos Orvostudomanyi Kar, Orvosi Mikrobiol Intezet, Debrecen, Hungary

来源：

ORVOSI HETILAP | 2014年 / 155卷 / 38期

关键词：

MALDI-TOF MS; species level identification; typing; antibiotic resistance determination; MALDI-TOF MS; BACTEROIDES-FRAGILIS STRAINS; GRAM-NEGATIVE BACTERIA; RAPID IDENTIFICATION; BRUKER BIOTYPER; DISCRIMINATION; MYCOBACTERIA; DIFFERENTIATION; MICROORGANISMS; RESISTANCE;

D O I：

10.1556/OH.2014.29985

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identifi cation of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identifi cation of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a rapid method which is able to identify a great variety of the isolated bacteria and fungi based on the composition of conserved ribosomal proteins. Recently several other

引用

页码：1495 / 1503

页数：9

共 50 条

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Schaumann Reiner, 2013, Open Microbiol J, V7, P118, DOI 10.2174/1874285801307010118

[32] Identification of Francisella tularensis by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Fast, Reliable, Robust, and Cost-Effective Differentiation on Species and Subspecies Levels [J].