Modulation of metalloproteinase-9 in U87MG glioblastoma cells by A3 adenosine receptors

被引：64
作者：
Gessi, Stefania [1 ,2 ]
Sacchetto, Valeria [1 ,2 ]
Fogli, Eleonora [1 ,2 ]
Merighi, Stefania [1 ,2 ]
Varani, Katia [1 ,2 ]
Baraldi, Pier Giovanni [3 ]
Tabrizi, Mojgan Aghazadeh [3 ]
Leung, Edward [4 ]
Maclennan, Stephen [4 ]
Borea, Pier Andrea [1 ,2 ]
机构：
[1] Univ Ferrara, Chair Pharmacol, Fac Med, Dept Clin & Expt Med,Pharmacol Unit, I-44100 Ferrara, Italy
[2] Univ Ferrara, Interdisciplinary Ctr Study Inflammat, I-44100 Ferrara, Italy
[3] Univ Ferrara, Dept Pharmaceut Sci, I-44100 Ferrara, Italy
[4] King Pharmaceut Res & Dev Inc, Cary, NC USA
来源：
BIOCHEMICAL PHARMACOLOGY | 2010年 / 79卷 / 10期
关键词：
A(3) adenosine receptors; MMP-9; Glioblastoma cells; Intracellular signalling; Cell invasion; ENDOTHELIAL GROWTH-FACTOR; MATRIX METALLOPROTEINASES; SIGNALING PATHWAYS; MATRIX-METALLOPROTEINASE-9; SECRETION; BIOCHEMICAL-CHARACTERIZATION; TISSUE INHIBITOR; GENE-EXPRESSION; UP-REGULATION; GELATINASE-B; KAPPA-B;
D O I：
10.1016/j.bcp.2010.01.009
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this work, we investigated the biological functions of adenosine (ado) in metalloproteinase-9 (MMP-9) regulation in U87MG human glioblastoma cells The nucleoside was able to increase both MMP-9 mRNA and protein levels through A(3) receptors activation We revealed that A(3) receptor stimulation induced an increase of MMP-9 protein levels in cellular extracts of U87MG cells by phosphorylation of extracellular signal-regulated protein kinases (ERK1/2), c-Jun N-terminal kinase/stress-activated protein kinase (pJNK/SAPK), protein kinase B (Akt/PKB) and finally activator protein 1 (AP-1) A(3) receptor activation stimulated also an increase of extracellular MMP-9 in the supernatants from U87MG glioblastoma cells Finally, the Matrigel invasion assay demonstrated that A(3) receptors, by inducing an increase in MMP-9 levels, was responsible for an increase of glioblastoma cells invasion Collectively, these results suggest that ado, through A(3) receptors activation, modulates MMP-9 protein levels and plays a role in increasing invasion of U87MG cells. (C) 2010 Elsevier Inc All rights reserved.
引用
收藏
页码：1483 / 1495
页数：13
相关论文
共 61 条
[1]   Derivation and characterization of a Wilms' tumour cell line, WiT 49 [J].
Alami, J ;
Williams, BR ;
Yeger, H .
INTERNATIONAL JOURNAL OF CANCER, 2003, 107 (03) :365-374
[2]   Secretion of matrix metalloproteinase-9 by the proinflammatory cytokine, IL-1β:: a role for the dual signalling pathways, Akt and Erk [J].
Amin, ARMR ;
Senga, T ;
Oo, ML ;
Thant, AA ;
Hamaguchi, M .
GENES TO CELLS, 2003, 8 (06) :515-523
[3]   Cloning, characterisation and chromosomal assignment of the human adenosine A3 receptor (ADORA3) gene [J].
Atkinson, MR ;
TownsendNicholson, A ;
Nicholl, JK ;
Sutherland, GR ;
Schofield, PR .
NEUROSCIENCE RESEARCH, 1997, 29 (01) :73-79
[4]   Post-transcriptional regulation in cancer [J].
Audic, Y ;
Hartley, RS .
BIOLOGY OF THE CELL, 2004, 96 (07) :479-498
[5]   The A3 adenosine receptor agonist CF102 induces apoptosis of hepatocellular carcinoma via de-regulation of the Wnt and NF-κB signal transduction pathways [J].
Bar-Yehuda, S. ;
Stemmer, S. M. ;
Madi, L. ;
Castel, D. ;
Ochaion, A. ;
Cohen, S. ;
Barer, F. ;
Zabutti, A. ;
Perez-Liz, G. ;
Del Valle, L. ;
Fishman, P. .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2008, 33 (02) :287-295
[6]   CF101, an agonist to the A3 adenosine receptor, enhances the chemotherapeutic effect of 5-fluorouracil in a colon carcinoma murine model [J].
Bar-Yehuda, S ;
Madi, L ;
Silberman, D ;
Gery, S ;
Shkapenuk, M ;
Fishman, P .
NEOPLASIA, 2005, 7 (01) :85-90
[7]   Adenosine receptor antagonists: Translating medicinal chemistry and pharmacology into clinical utility [J].
Baraldi, Pier Giovanni ;
Tabrizi, Mojgan Aghazadeh ;
Gessi, Stefania ;
Borea, Pier Andrea .
CHEMICAL REVIEWS, 2008, 108 (01) :238-263
[8]   SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase [J].
Bennett, BL ;
Sasaki, DT ;
Murray, BW ;
O'Leary, EC ;
Sakata, ST ;
Xu, WM ;
Leisten, JC ;
Motiwala, A ;
Pierce, S ;
Satoh, Y ;
Bhagwat, SS ;
Manning, AM ;
Anderson, DW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (24) :13681-13686
[9]   Regulation of matrix metalloproteinases: An overview [J].
Chakraborti, S ;
Mandal, M ;
Das, S ;
Mandal, A ;
Chakraborti, T .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2003, 253 (1-2) :269-285
[10]   Long-Range Enhancer Associated with Chromatin Looping Allows AP-1 Regulation of the Peptidylarginine Deiminase 3 Gene in Differentiated Keratinocyte [J].
Chavanas, Stephane ;
Adoue, Veronique ;
Mechin, Marie-Claire ;
Ying, Shibo ;
Dong, Sijun ;
Duplan, Helene ;
Charveron, Marie ;
Takahara, Hidenari ;
Serre, Guy ;
Simon, Michel .
PLOS ONE, 2008, 3 (10)
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