Association of several small heat-shock proteins with reproductive tissues in the nematode Caenorhabditis elegans

被引:17
作者
Ding, L [1 ]
Candido, EPM [1 ]
机构
[1] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z3, Canada
关键词
alpha-crystallin; immunocytochemistry; tissue specificity;
D O I
10.1042/0264-6021:3510013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Immunohistochemical data on 10 of the 14 small heat-shock (smHSPs) proteins in fourth larval stage and adult Caenorhabditis elegans show that the tissues expressing the greatest number of smHSPs are vulva (HSP12s, HSP43 and, under stress, HSP16s) and spermatheca (HSP12s, HSP25, HSP43 and, under stress, HSP16s). HSP43 is also expressed in male tail structures, and following heat-shock HSP16s are expressed in spermatids and spermatozoa.
引用
收藏
页码:13 / 17
页数:5
相关论文
共 26 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Localization of heat shock proteins in mouse male germ cells: An immunoelectron microscopical study [J].
Biggiogera, M ;
Tanguay, RM ;
Marin, R ;
Wu, Y ;
Martin, TE ;
Fakan, S .
EXPERIMENTAL CELL RESEARCH, 1996, 229 (01) :77-85
[3]  
BRENNER S, 1974, GENETICS, V77, P71
[4]   HSP25, a small heat shock protein associated with dense bodies and M-lines of body wall muscle in Caenorhabditis elegans [J].
Ding, L ;
Candido, EPM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (13) :9510-9517
[5]   HSP43, a small heat-shock protein localized to specific cells of the vulva and spermatheca in the nematode Caenorhabditis elegans [J].
Ding, L ;
Candido, EPM .
BIOCHEMICAL JOURNAL, 2000, 349 :409-412
[6]   THE UNC-86 GENE-PRODUCT COUPLES CELL LINEAGE AND CELL IDENTITY IN C-ELEGANS [J].
FINNEY, M ;
RUVKUN, G .
CELL, 1990, 63 (05) :895-905
[7]   STUDIES OF THE SMALL HEAT-SHOCK PROTEINS OF CAENORHABDITIS-ELEGANS USING ANTIPEPTIDE ANTIBODIES [J].
HOCKERTZ, MK ;
CLARKLEWIS, I ;
CANDIDO, EPM .
FEBS LETTERS, 1991, 280 (02) :375-378
[8]   DIFFERENTIAL REGULATION OF CLOSELY RELATED MEMBERS OF THE HSP16 GENE FAMILY IN CAENORHABDITIS-ELEGANS [J].
JONES, D ;
DIXON, DK ;
GRAHAM, RW ;
CANDIDO, EPM .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (07) :481-490
[9]   Transgenic strains of the nematode C-elegans in biomonitoring and toxicology: Effects of captan and related compounds on the stress response [J].
Jones, D ;
Stringham, EG ;
Babich, SL ;
Candido, EPM .
TOXICOLOGY, 1996, 109 (2-3) :119-127
[10]  
JONES D, 1986, J BIOL CHEM, V261, P2006