Characterization of Bacterial Cellulose Produced by Komagataeibacter maltaceti P285 Isolated from Contaminated Honey Wine

被引:23
作者
Thongwai, Narumol [1 ,2 ]
Futui, Wirapong [1 ,3 ]
Ladpala, Nanthiwa [1 ]
Sirichai, Benjamat [1 ]
Weechan, Anuwat [1 ]
Kanklai, Jirapat [1 ,3 ]
Rungsirivanich, Patthanasak [1 ,3 ]
机构
[1] Chiang Mai Univ, Fac Sci, Dept Biol, Chiang Mai 50200, Thailand
[2] Chiang Mai Univ, Res Ctr Bioresources Agr Ind & Med, Chiang Mai 50200, Thailand
[3] Chiang Mai Univ, Grad Sch, Chiang Mai 50200, Thailand
关键词
Acetobacter xylinum; biopolymer; honey; mead; optimization; sugarcane; Novacetimonas; GLUCONACETOBACTER-MALTACETI; EXTRACTION; MEDIA; PH; ACIDS; RICE; NOV; DNA;
D O I
10.3390/microorganisms10030528
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family Acetobacteraceae. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with Komagataeibacter maltaceti LMG 1529 (T). K. maltaceti P285 produced the maximum BC production at 20-30 degrees C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, w/v) and yeast extract (3.2%, w/v) were applied as carbon and nitrogen sources, respectively. When sugarcane (8-16%, w/v) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by K. maltaceti P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2 theta = 18.330, 21.390 and 22.640 degrees). The maximum temperature of BC degradation was observed at 314 degrees C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that K. maltaceti P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories.
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页数:18
相关论文
共 76 条
[1]   Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions [J].
Alemam, Ahmed M. ;
Shaheen, Tharwat, I ;
Hassan, Saad El-Din ;
Desouky, Said E. ;
El-Gamal, Mamdouh S. .
EGYPTIAN JOURNAL OF CHEMISTRY, 2021, 64 (04) :2213-2221
[2]   Pineapple Agroindustrial Residues for the Production of High Value Bacterial Cellulose with Different Morphologies [J].
Algar, Itxaso ;
Fernandes, Susana C. M. ;
Mondragon, Gurutz ;
Castro, Cristina ;
Garcia-Astrain, Clara ;
Gabilondo, Nagore ;
Retegi, Alona ;
Eceiza, Arantxa .
JOURNAL OF APPLIED POLYMER SCIENCE, 2015, 132 (01)
[3]   New biotextiles for tissue engineering: Development, characterization and in vitro cellular viability [J].
Almeida, Lilia R. ;
Martins, Ana R. ;
Fernandes, Emanuel M. ;
Oliveira, Mariana B. ;
Correlo, Vitor M. ;
Pashkuleva, Iva ;
Marques, Alexandra P. ;
Ribeiro, Ana S. ;
Duraes, Nelson F. ;
Silva, Carla J. ;
Bonifacio, Graca ;
Sousa, Rui A. ;
Oliveira, Ana L. ;
Reis, Rui L. .
ACTA BIOMATERIALIA, 2013, 9 (09) :8167-8181
[4]   Mineral content and physical properties of local and imported honeys in Saudi Arabia [J].
Alqarni, Abdulaziz S. ;
Owayss, Ayman A. ;
Mahmoud, Awad A. ;
Hannan, Mohammed A. .
JOURNAL OF SAUDI CHEMICAL SOCIETY, 2014, 18 (05) :618-625
[5]   Scanning Electron Microscopy of Bacteria Tetrasphaera duodecadis [J].
Arroyo, E. ;
Enriquez, L. ;
Sanchez, A. ;
Ovalle, M. ;
Olivas, A. .
SCANNING, 2014, 36 (05) :547-550
[6]   Optimized culture conditions for bacterial cellulose production byAcetobacter senegalensisMA1 [J].
Aswini, K. ;
Gopal, N. O. ;
Uthandi, Sivakumar .
BMC BIOTECHNOLOGY, 2020, 20 (01)
[7]   Raman and FT-IR Spectroscopy investigation the cellulose structural differences from bacteria Gluconacetobacter sucrofermentans during the different regimes of cultivation on a molasses media [J].
Atykyan, Nelli ;
Revin, Victor ;
Shutova, Vitalina .
AMB EXPRESS, 2020, 10 (01)
[8]  
Autal R., 2017, AFR J BIOTECHNOL, V16, P470, DOI [DOI 10.5897/AJB2016.15486, 10.5897/AJB2016, DOI 10.5897/AJB2016]
[9]   Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications [J].
Azeredo, Henriette M. C. ;
Barud, Hernane ;
Farinas, Cristiane S. ;
Vasconcellos, Vanessa M. ;
Claro, Amanda M. .
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 2019, 3
[10]   Characterization of Bacterial Cellulose Produced using Media Containing Waste Apple Juice [J].
Bandyopadhyay, S. ;
Saha, N. ;
Saha, P. .
APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2018, 54 (06) :649-657