Structural Insights into the Mechanism of Scanning and Start Codon Recognition in Eukaryotic Translation Initiation

被引：214

作者：

Hinnebusch, Alan G. ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Lab Gene Regulat & Dev, NIH, Bethesda, MD 20892 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2017年 / 42卷 / 08期

关键词：

40S RIBOSOMAL-SUBUNIT; MESSENGER-RNA RECRUITMENT; C-TERMINAL DOMAIN; FACTOR; EIF3; P-I RELEASE; MULTIFACTOR COMPLEX; CRYSTAL-STRUCTURE; CONFORMATIONAL-CHANGES; YEAST; BINDING;

D O I：

10.1016/j.tibs.2017.03.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Initiation of translation on eukaryotic mRNAs generally follows the scanning mechanism, wherein a preinitiation complex (PIC) assembled on the small (40S) ribosomal subunit and containing initiator methionyl tRNAi (Met-tRNAi) scans the mRNA leader for an AUG codon. In a current model, the scanning PIC adopts an open conformation and rearranges to a closed state, with fully accommodated Met-tRNAi, upon AUG recognition. Evidence from recent high-resolution structures of PICs assembled with different ligands supports this model and illuminates the molecular functions of eukaryotic initiation factors eIF1, eIF1A, and eIF2 in restricting to AUG codons the transition to the closed conformation. They also reveal that the eIF3 complex interacts with multiple functional sites in the PIC, rationalizing its participation in numerous steps of initiation.

引用

页码：589 / 611

页数：23

共 93 条

[1] Reconstitution of yeast translation initiation [J].

Acker, Michael G. ;

Kolitz, Sarah E. ;

Mitchell, Sarah F. ;

Nanda, Jagpreet S. ;

Lorsch, Jon R. .

TRANSLATION INITIATION: RECONSTITUTED SYSTEMS AND BIOPHYSICAL METHODS, 2007, 430 :111-145

[2] Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex [J].

Aitken, Colin Echeverria ;

Beznoskova, Petra ;

Vlckova, Vladislava ;

Chiu, Wen-Ling ;

Zhou, Fujun ;

Valasek, Leos Shivaya ;

Hinnebusch, Alan G. ;

Lorsch, Jon R. .

ELIFE, 2016, 5

[3] Pi release from elF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation [J].

Algire, MA ;

Maag, D ;

Lorsch, JR .

MOLECULAR CELL, 2005, 20 (02) :251-262

[4] A multifactor complex of eukaryotic initiation factors, eIE1, eIF2, eIF3, eIF5, and initiator tRNAMet is an important translation initiation intermediate in vivo [J].

Asano, K ;

Clayton, J ;

Shalev, A ;

Hinnebusch, AG .

GENES & DEVELOPMENT, 2000, 14 (19) :2534-2546

[5] Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex [J].

Aylett, Christopher H. S. ;

Boehringer, Daniel ;

Erzberger, Jan P. ;

Schaefer, Tanja ;

Ban, Nenad .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2015, 22 (03) :269-271

[6] The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons [J].

Chiu, Wen-Ling ;

Wagner, Susan ;

Herrmannova, Anna ;

Burela, Laxminarayana ;

Zhang, Fan ;

Saini, Adesh K. ;

Valasek, Leos ;

Hinnebusch, Alan G. .

MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (18) :4415-4434

[7] Cryo-EM study of start codon selection during archaeal translation initiation [J].

Coureux, Pierre-Damien ;

Lazennec-Schurdevin, Christine ;

Monestier, Auriane ;

Larquet, Eric ;

Cladiere, Lionel ;

Klaholz, Bruno P. ;

Schmitt, Emmanuelle ;

Mechulam, Yves .

NATURE COMMUNICATIONS, 2016, 7

[8] The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning [J].

Cuchalova, Lucie ;

Kouba, Tomas ;

Herrmannova, Anna ;

Danyi, Istvan ;

Chiu, Wen-ling ;

Valasek, Leos .

MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (19) :4671-4686

[9] Structure of mammalian eIF3 in the context of the 43S preinitiation complex [J].

des Georges, Amedee ;

Dhote, Vidya ;

Kuhn, Lauriane ;

Hellen, Christopher U. T. ;

Pestova, Tatyana V. ;

Frank, Joachim ;

Hashem, Yaser .

NATURE, 2015, 525 (7570) :491-+

[10]

Donahue TF, 2000, COLD SPRING HARBOR M, V39, P487

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