Hydrostatic pressure promotes migration and filamin-A activation in fibroblasts with increased p38 phosphorylation and TGF-β production

被引：2

作者：

Kao, Yu-Chiu ^{[1
,2
]}

Chen, Zih-Hua ^{[1
]}

Wang, Wen-Yu ^{[1
]}

Lee, Chau-Hwang ^{[2
,3
]}

Kuo, Po-Ling ^{[1
,4
,5
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat, Taipei 10617, Taiwan

[2] Acad Sinica, Res Ctr Appl Sci, Taipei 11529, Taiwan

[3] Natl Yang Ming Chiao Tung Univ, Inst Biophoton, Taipei 11221, Taiwan

[4] Natl Taiwan Univ, Dept Elect Engn, Taipei 10617, Taiwan

[5] Natl Taiwan Univ Hosp, Dept Rehabil, Taipei 10002, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 568卷

关键词：

Fibroblast; Interstitial hydrostatic pressure; Filamin; p38; TGF-beta; Cell motility; INTERSTITIAL FLUID PRESSURE; ENDOTHELIAL-CELLS; KINASE; CANCER; ALPHA; DIFFERENTIATION; PROLIFERATION; EXPRESSION; RESPONSES; MOTILITY;

D O I：

10.1016/j.bbrc.2021.06.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fibroblast migration is closely regulated by the mechanical characteristics in surrounding microenvironment. While increased interstitial hydrostatic pressure (HP) is a hallmark in many pathological and physiological conditions, little is known about how the HP affects fibroblast motility. Using cell-culture chips with elevated HP conditions, we showed that 20 cmH(2)O HP significantly accelerated fibroblast migration. The HP-induced migration acceleration was dependent on the augmentation of transforming growth factor-beta(1), and correlated with the activation of filamin A via the phosphorylation of p38 mitogen-activated protein kinase. Our results suggest that interstitial HP elevation associated with various pathological states could significantly regulate fibroblast migration. (C) 2021 The Authors. Published by Elsevier Inc.

引用

页码：15 / 22

页数：8

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