Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains

被引：116
作者：
Cerna, JF
Nataro, JP
Estrada-Garcia, T
机构：
[1] Inst Politecn Nacl, CINVESTAV, Dept Mol Biomed, Mexico City 07360, DF, Mexico
[2] Univ Maryland, Sch Med, Dept Pediat & Med, Ctr Vaccine Dev, Baltimore, MD 21201 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 05期
关键词：
D O I：
10.1128/JCM.41.5.2138-2140.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.
引用
收藏
页码：2138 / 2140
页数：3
相关论文
共 24 条
[1]  
Ausubel FA, 1995, CURRENT PROTOCOLS MO
[2]   A SENSITIVE AND SPECIFIC DNA PROBE TO IDENTIFY ENTEROAGGREGATIVE ESCHERICHIA-COLI, A RECENTLY DISCOVERED DIARRHEAL PATHOGEN [J].
BAUDRY, B ;
SAVARINO, SJ ;
VIAL, P ;
KAPER, JB ;
LEVINE, MM .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (06) :1249-1251
[3]   ENTEROAGGREGATIVE ESCHERICHIA-COLI AND SALMONELLA ASSOCIATED WITH NONDYSENTERIC PERSISTENT DIARRHEA [J].
BHAN, MK ;
KHOSHOO, V ;
SOMMERFELT, H ;
RAJ, P ;
SAZAWAL, S ;
SRIVASTAVA, R .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1989, 8 (08) :499-502
[4]   Short report:: Characterization of enteroaggregative Escherichia coli isolates from Iranian children [J].
Bouzari, S ;
Jafari, A ;
Azizi, A ;
Oloomi, M ;
Nataro, JP .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2001, 65 (01) :13-14
[5]   ASSOCIATION OF ESCHERICHIA-COLI HEP-2 ADHERENCE PATTERNS WITH TYPE AND DURATION OF DIARRHEA [J].
CRAVIOTO, A ;
TELLO, A ;
NAVARRO, A ;
RUIZ, J ;
VILLAFAN, H ;
URIBE, F ;
ESLAVA, C .
LANCET, 1991, 337 (8736) :262-264
[6]   ADHESIVE FACTOR FOUND IN STRAINS OF ESCHERICHIA-COLI BELONGING TO THE TRADITIONAL INFANTILE ENTEROPATHOGENIC SEROTYPES [J].
CRAVIOTO, A ;
GROSS, RJ ;
SCOTLAND, SM ;
ROWE, B .
CURRENT MICROBIOLOGY, 1979, 3 (02) :95-99
[7]   Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli [J].
Czeczulin, JR ;
Whittam, TS ;
Henderson, IR ;
Navarro-Garcia, F ;
Nataro, JP .
INFECTION AND IMMUNITY, 1999, 67 (06) :2692-2699
[8]   Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli [J].
Eslava, C ;
Navarro-García, F ;
Czeczulin, JR ;
Henderson, IR ;
Cravioto, A ;
Nataro, JP .
INFECTION AND IMMUNITY, 1998, 66 (07) :3155-3163
[9]   ETIOLOGY AND EPIDEMIOLOGY OF PERSISTENT DIARRHEA IN NORTHEASTERN BRAZIL - A HOSPITAL-BASED, PROSPECTIVE, CASE-CONTROL STUDY [J].
FANG, GD ;
LIMA, AAM ;
MARTINS, CV ;
NATARO, JP ;
GUERRANT, RL .
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1995, 21 (02) :137-144
[10]   Rate of occurrence and pathogenic effect of enteroaggregative Escherichia coli virulence factors in international travelers [J].
Jiang, ZD ;
Greenberg, D ;
Nataro, JP ;
Steffen, R ;
DuPont, HL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4185-4190
123