Mechanistic investigation of ionization suppression in electrospray ionization

被引：930

作者：

King, R ^{[1
]}

Bonfiglio, R ^{[1
]}

Fernandez-Metzler, C ^{[1
]}

Miller-Stein, C ^{[1
]}

Olah, T ^{[1
]}

机构：

[1] Merck Res Labs, Dept Drug Metab, W Point, PA 19486 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2000年 / 11卷 / 11期

关键词：

D O I：

10.1016/S1044-0305(00)00163-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We show results from experiments designed to determine the relative importance of gas phase processes and solution phase processes into ionization suppression observed in biological sample extracts. The data indicate that gas phase reactions leading to the loss of net charge on the analyte is not Likely to be the most important process involved in ionization suppression. The results point to changes in the droplet solution properties caused by the presence of nonvolatile solutes as the main cause of ionization suppression in electrospray ionization of biological extracts. (C) 2000 American Society for Mass Spectrometry.

引用

页码：942 / 950

页数：9

共 21 条

[1] ULTRASONICALLY ASSISTED ELECTROSPRAY-IONIZATION FOR LC/MS DETERMINATION OF NUCLEOSIDES FROM A TRANSFER-RNA DIGEST [J].