The optical and molecular signatures of DOM under the eutrophication status in a shallow, semi-enclosed coastal bay in southeast China

We applied stable carbon isotopes, ultraviolet-visible absorption (UV-Vis) and fluorescence excitation-emission matrices spectroscopy (EEMs), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to investigate the chemical composition and sources of the dissolved organic matter (DOM) in both the water column and pore water in Xiangshan Bay, a representative semi-enclosed and eutrophic bay in Zhejiang Province, China. One protein-like fluorescent component (C1) and two humic-like fluorescent components (C2 and C3) were identified by PARAFAC modeling. The concentration of dissolved organic carbon (DOC), the relative intensities of C2, C3, and black carbon-like compounds are all negatively correlated with salinity, indicating that there is a dilution effect of terrestrial signals by seawater in Xiangshan Bay. The differences in light penetration ability of Xiangshan Bay cause different degrees of photo-degradation, which may play an important role in the transformation of organic matter in Xiangshan Bay. The weak correlation between the C1 fluorescent component and salinity indicates that autochthonous sources cannot dominate the protein-like FDOM in the Xiangshan Bay drainage area. Multiple sources (such as anthropogenic inputs and release of pore water) also affect the distribution of the protein-like fluorescent component under eutrophication conditions. The relative proportion of the protein-like fluorescent component in Xiangshan Bay is on a medium level in China and anthropogenic inputs may be a significant source of DOM in coastal bays.