Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume

被引：39
|
作者：
Padmanabhan, Saraswathi [2 ]
Shinoj, Vengalathunadakal K. [2 ]
Murukeshan, Vadakke M. [2 ]
Padmanabhan, Parasuraman [1 ]
机构：
[1] Singapore BioImaging Consortium, Lab Mol Imaging, Singapore 138667, Singapore
[2] Nanyang Technol Univ, Sch Mech & Aerosp Engn, Singapore 639798, Singapore
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 01期
关键词：
fiber optic sensor; photonic crystal fiber; MCF-7; cells; protein immobilization; estrogen receptor; fluorescence imaging and sensing; ESTROGEN-RECEPTOR; BIOSENSORS; ASSAY;
D O I：
10.1117/1.3302810
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor (ER) from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either Alexa (TM) Fluor 488 (green fluorescent dye) or 555 (red Fluorescent dye) labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ER alpha protein were observed under fluorescence microscope, and its optical characteristics were analyzed. The ER alpha protein detection by this proposed method is based on immuno binding from sample volume as low as 50 nL. This method is expected to offer great potential as a biosensor for medical diagnostics and therapeutics applications. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3302810]
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页数：6
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