Analysis of nif and regulatory genes in Acetobacter diazotrophicus

被引:15
|
作者
Sevilla, M
Meletzus, D
Teixeira, K
Lee, S
Nutakki, A
Baldani, I
Kennedy, C
机构
[1] UNIV ARIZONA,DEPT PLANT PATHOL,TUCSON,AZ 85721
[2] UNIV BIELEFELD,FAC BIOL,DEPT GENE TECHNOL & MICROBIOL,D-33501 BIELEFELD,GERMANY
[3] EMBRAPA,CNPBS,BR-23851 RIO JANEIRO,BRAZIL
来源
SOIL BIOLOGY & BIOCHEMISTRY | 1997年 / 29卷 / 5-6期
关键词
D O I
10.1016/S0038-0717(96)00210-6
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:871 / 874
页数:4
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