A novel red emission fluorescent probe for monitoring carbon monoxide in living cells and zebrafish

被引：11

作者：

Chen, Jie ^{[1
]}

Gan, Yabing ^{[1
]}

Hong, Sai ^{[1
]}

Yin, Guoxing ^{[1
]}

Zhou, Li ^{[2
,3
]}

Wang, Changze ^{[4
]}

Fu, Yuchuan ^{[2
,3
]}

Li, Haitao ^{[1
]}

Yin, Peng ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China

[2] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Dept Radiat Oncol, Chengdu 610041, Peoples R China

[3] Sichuan Univ, West China Hosp, Canc Ctr, Chengdu 610041, Peoples R China

[4] Shangyu NHU BIOchem CO LTD, Shangyu, Peoples R China

来源：

ANALYTICAL METHODS | 2021年 / 13卷 / 25期

基金：

中国国家自然科学基金;

关键词：

CO; HEME;

D O I：

10.1039/d1ay00704a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Carbon monoxide (CO), a gaseous signal molecule, plays a crucial role in biological systems. With the aim of unraveling its biological functions, a novel fluorescent probe for sensing CO was rationally designed and synthesized based on a coumarin derivative fluorophore merging tetrahydroquinoxaline unit and five-membered pyrrolidine. This fluorescent probe demonstrated a large Stokes shift (Delta lambda = 132 nm), high quantum yield, red emission, high sensitivity and selectivity for CO with remarkable fluorescence turn-on. And the detection limit for CORM-3 is as low as 31.2 nM with the linear range of 0-30 mu M. More importantly, this novel probe has been successfully applied to the fluorescence imaging of CO in HepG2 cells and zebrafish, providing a useful approach for the further understanding of the physiological and pathological roles of CO in living systems.

引用

页码：2871 / 2877

页数：7

共 54 条

[1] Bench-to-bedside review: Carbon monoxide - from mitochondrial poisoning to therapeutic use [J].

Bauer, Inge ;

Pannen, Benedikt H. J. .

CRITICAL CARE, 2009, 13 (04)

[2] Stereospecific and Reversible CO Binding at Iron Pincer Complexes [J].

Benito-Garagorri, David ;

Puchberger, Michael ;

Mereiter, Kurt ;

Kirchner, Karl .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (47) :9142-9145

[3] Cardioprotective actions by a water-soluble carbon monoxide-releasing molecule [J].

Clark, JE ;

Naughton, P ;

Shurey, S ;

Green, CJ ;

Johnson, TR ;

Mann, BE ;

Foresti, R ;

Motterlini, R .

CIRCULATION RESEARCH, 2003, 93 (02) :E2-E8

[4] The measurement of endogenous carbon monoxide production [J].

Coburn, Ronald F. .

JOURNAL OF APPLIED PHYSIOLOGY, 2012, 112 (11) :1949-1955

[5] Ex Vivo Tracking of Endogenous CO with a Ruthenium(II) Complex [J].

de la Torre, Cristina ;

Toscani, Anita ;

Marin-Hernandez, Cristina ;

Robson, Jonathan A. ;

Carmen Terencio, Maria ;

White, Andrew J. P. ;

Alcaraz, Maria Jose ;

Wilton-Ely, James D. E. T. ;

Martinez-Manez, Ramon ;

Sancenon, Felix .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (51) :18484-18487

[6] A nano-cocktail of an NIR-II emissive fluorophore and organoplatinum(ii) metallacycle for efficient cancer imaging and therapy [J].

Ding, Feng ;

Chen, Zhao ;

Kim, Won Young ;

Sharma, Amit ;

Li, Chonglu ;

Ouyang, Qingying ;

Zhu, Hua ;

Yang, Guangfu ;

Sun, Yao ;

Kim, Jong Seung .

CHEMICAL SCIENCE, 2019, 10 (29) :7023-7028

[7]

Douglas CG, 1912, J PHYSIOL-LONDON, V44, P275

[8] Sensitive and Selective Chromogenic Sensing of Carbon Monoxide by Using Binuclear Rhodium Complexes [J].

Esteban, Julio ;

Vicente Ros-Lis, Jose ;

Martinez-Manez, Ramon ;

Dolores Marcos, M. ;

Moragues, Maria ;

Soto, Juan ;

Sancenon, Felix .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (29) :4934-4937

[9] A fluorescent probe for carbon monoxide based on allyl ether rather than allyl ester: A practical strategy to avoid the interference of esterase in cell imaging [J].

Fang, Wen-Le ;

Tang, Ying-Jie ;

Guo, Xiao-Feng ;

Wang, Hong .

TALANTA, 2019, 205

[10] Ratiometric fluorescent probe for sensing Streptococcus mutans glucosyltransferase, a key factor in the formation of dental caries [J].

Feng, Lei ;

Yan, Qingsong ;

Zhang, Baojing ;

Tian, Xiangge ;

Wang, Chao ;

Yu, Zhenlong ;

Cui, Jingnan ;

Guo, Dean ;

Ma, Xiaochi ;

James, Tony D. .

CHEMICAL COMMUNICATIONS, 2019, 55 (24) :3548-3551

← 1 2 3 4 5 6 →