Response surface methodology based optimization of keratinase production from Trichoderma harzianum isolate HZN12 using chicken feather waste and its application in dehairing of hide

被引:50
作者
Bagewadi, Zabin K. [1 ,2 ]
Mulla, Sikandar I. [2 ,3 ]
Ninnekar, Harichandra Z. [2 ]
机构
[1] KLE Technol Univ, Dept Biotechnol, Hubballi 580031, Karnataka, India
[2] Karnatak Univ, Dept Biochem, Dharwad 580003, Karnataka, India
[3] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Pollutant Convers, Xiamen, Peoples R China
来源
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING | 2018年 / 6卷 / 04期
关键词
Keratinase; Trichoderma harzianum; Chicken feather; Response surface methodology; Dehairing; Detergent stability; KERATINOLYTIC PROTEASE; EXPERIMENTAL-DESIGN; BIODEGRADATION; PURIFICATION; DETERGENT; DEGRADATION; FUNGI;
D O I
10.1016/j.jece.2018.07.007
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Keratin proteins are a major constituent of feathers which largely contribute to environmental pollution. The present study reports a potent feather degrading fungi isolated from soil and molecularly characterized (18S rDNA gene sequencing) and identified as Trichoderma harzianum isolate HZN12 (KP235366). Assessment of keratinase production from the isolate revealed better production under solid state fermentation (SSF) conditions (1320 U/g) over submerged (185 U) using chicken feathers. The effect and contribution of independent process variables were studied by Plackets-Burman design. Additionally, statistical modeling by central composite design of response surface methodology was applied to optimize the culture conditions for keratinase production under SSF. Maximum keratinase production (10,150 U/g) was validated with a 7.7 fold increase under the optimized conditions (chicken feather meal 3 g/20 ml, yeast extract 0.2%, glucose 0.9%, pH 8.5 and temperature of 37 degrees C) with a correlation coefficient (R-2) = 95.56%. Keratinase was employed for dehairing of goat hide and assessed based on different organoleptic properties. The keratinase dehaired skin exhibited superior properties in comparison to the chemical treatment. Furthermore, keratinase also showed good stability and compatibility with the tested commercial detergents making it suitable for industrial applications such as, leather and detergent industries. The results suggest an ecofriendly approach for biodegradation of chicken feather waste.
引用
收藏
页码:4828 / 4839
页数:12
相关论文
共 49 条
[1]   Structural characterization, catalytic, kinetic and thermodynamic properties of Keratinase from Bacillus pumilus FH9 [J].
Abdel-Naby, Mohamed A. ;
El-Refai, Heba A. ;
Ibrahim, Mohammad H. A. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 105 :973-980
[2]   Extracellular keratinase from Trichophyton sp HA-2 isolated from feather dumping soil [J].
Anbu, Periasamy ;
Hilda, Azariah ;
Sur, Hwal-Won ;
Hur, Byung-Ki ;
Jayanthi, S. .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2008, 62 (03) :287-292
[3]   Purification and characterization of an extracellular keratinolytic protease from a new isolate of Aspergillus parasiticus [J].
Anitha, T. S. ;
Palanivelu, P. .
PROTEIN EXPRESSION AND PURIFICATION, 2013, 88 (02) :214-220
[4]  
[Anonymous], 1946, Biometrika, DOI [DOI 10.2307/2332195, 10.2307/2332195, DOI 10.1093/BIOMET/33.4.305]
[5]   Optimization of endoglucanase production from Trichoderma harzianum strain HZN11 by central composite design under response surface methodology [J].
Bagewadi, Zabin K. ;
Mulla, Sikandar I. ;
Ninnekar, Harichandra Z. .
BIOMASS CONVERSION AND BIOREFINERY, 2018, 8 (02) :305-316
[6]  
Bagewadi ZK, 2017, INT J RECYCLING ORG, V6, P351, DOI 10.1007/s40093-017-0184-4
[7]   Xylanase production from Penicillium citrinum isolate HZN13 using response surface methodology and characterization of immobilized xylanase on glutaraldehyde-activated calcium-alginate beads [J].
Bagewadi, Zabin K. ;
Mulla, Sikandar I. ;
Shouche, Yogesh ;
Ninnekar, Harichandra Z. .
3 BIOTECH, 2016, 6
[8]   Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace [J].
Belmessikh, Aicha ;
Boukhalfa, Hayet ;
Mechakra-Maza, Aicha ;
Gheribi-Aoulmi, Zoubida ;
Amrane, Abdeltif .
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS, 2013, 44 (03) :377-385
[9]  
Bohacz J., 2017, WORLD J MICROB BIOT, P33
[10]   Novel serine keratinase from Caldicoprobacter algeriensis exhibiting outstanding hide dehairing abilities [J].
Bouacem, Khelifa ;
Bouanane-Darenfed, Amel ;
Jaouadib, Nadia Zarai ;
Joseph, Manon ;
Hacene, Hocine ;
Ollivier, Bernard ;
Fardeau, Marie-Laure ;
Bejar, Samir ;
Jaouadi, Bassem .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 86 :321-328