Indigo Naturalis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating the Intestinal Microbiota Community

被引:64
作者
Liang, Yan-Ni [1 ]
Yu, Jin-Gao [1 ]
Zhang, Dong-Bo [1 ]
Zhang, Zhen [1 ]
Ren, Lang-Lang [1 ]
Li, Lu-Han [1 ]
Wang, Zheng [1 ]
Tang, Zhi-Shu [1 ]
机构
[1] Shaanxi Univ Chinese Med, Shaanxi Innovat Drug Res Ctr, Shaanxi Collaborat Innovat Ctr Chinese Med Resour, State Key Lab Res & Dev Characterist Qin Med Reso, Xian Yang 712083, Peoples R China
基金
中国国家自然科学基金;
关键词
indigo naturalis; colitis; DSS; gut microbiota; 16S rDNA; INFLAMMATORY-BOWEL-DISEASE; CHINESE HERBAL MEDICINE; ULCERATIVE-COLITIS; GUT MICROBIOTA; XILEI-SAN; QING-DAI; HOST; COLONIZATION; INDIRUBIN; SEQUENCES;
D O I
10.3390/molecules24224086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan. Though there are notable effects of IN on colitis, the mechanisms remain elusive. Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota. To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions. Sulfasalazine (SASP) was used as the positive control. Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC). The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines. The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus. The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus. The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.
引用
收藏
页数:17
相关论文
共 65 条
[1]   Indirubin and indigo are potent aryl hydrocarbon receptor ligands present in human urine [J].
Adachi, J ;
Mori, Y ;
Matsui, S ;
Takigami, H ;
Fujino, J ;
Kitagawa, H ;
Miller, CA ;
Kato, T ;
Saeki, K ;
Matsuda, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (34) :31475-31478
[2]   Apigenin inhibits colonic inflammation and tumorigenesis by suppressing STAT3-NF-κB signaling [J].
Ai, Xiao-Yu ;
Qin, Yuan ;
Liu, Hui-Jua ;
Cui, Zhan-Hong ;
Li, Meng ;
Yang, Jia-Huan ;
Zhong, Wei-Long ;
Liu, Yan-Rong ;
Chen, Shuang ;
Sun, Tao ;
Zhou, Hong-Gang ;
Yang, Cheng .
ONCOTARGET, 2017, 8 (59) :100216-100226
[3]   Fusobacterium nucleatum, inflammation, and immunity: the fire within human gut [J].
Bashir, Arif ;
Miskeen, Abid Yousuf ;
Hazari, Younis Mohammad ;
Asrafuzzaman, Syed ;
Fazili, Khalid Majid .
TUMOR BIOLOGY, 2016, 37 (03) :2805-2810
[4]   Th22 Cells Are an Important Source of IL-22 for Host Protection against Enteropathogenic Bacteria [J].
Basu, Rajatava ;
O'Quinn, Darrell B. ;
Silberger, Daniel J. ;
Schoeb, Trenton R. ;
Fouser, Lynette ;
Ouyang, Wenjun ;
Hatton, Robin D. ;
Weaver, Casey T. .
IMMUNITY, 2012, 37 (06) :1061-1075
[5]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[6]  
Bosshard PP, 2002, INT J SYST EVOL MICR, V52, P1263, DOI 10.1099/00207713-52-4-1263
[7]  
Brook I., 2017, ANAEROB BACT, V2, P1628
[8]  
COOPER HS, 1993, LAB INVEST, V69, P238
[9]  
de Souza HSP, 2016, NAT REV GASTRO HEPAT, V13, P13, DOI [10.1038/nrgastro.2015.186, 10.1038/nrgastro.2016.186]
[10]  
Edgar RC, 2013, NAT METHODS, V10, P996, DOI [10.1038/NMETH.2604, 10.1038/nmeth.2604]