Nitrogen supply rate regulates microbial resource allocation for synthesis of nitrogen-acquiring enzymes

被引:14
|
作者
Fujita, Kazuki [1 ]
Kunito, Takashi [1 ]
Matsushita, Junko [1 ]
Nakamura, Kaori [1 ]
Moro, Hitoshi [1 ]
Yoshida, Seishi [2 ]
Toda, Hideshige [1 ]
Otsuka, Shigeto [3 ]
Nagaoka, Kazunari [4 ,5 ]
机构
[1] Shinshu Univ, Fac Sci, Dept Environm Sci, Matsumoto, Nagano, Japan
[2] Nagano Prefecture Vegetable & Ornamental Crops Ex, Shiojiri, Japan
[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo, Japan
[4] NARO, Cent Reg Agr Res Ctr, Tsukuba, Ibaraki, Japan
[5] NARO, Hokkaido Agr Res Ctr, Sapporo, Hokkaido, Japan
来源
PLOS ONE | 2018年 / 13卷 / 08期
关键词
L-ASPARAGINASE; SACCHAROMYCES-CEREVISIAE; CATABOLITE REPRESSION; NUTRIENT LIMITATION; SOIL; PHOSPHORUS; INDEXES; MICROORGANISMS; FERTILIZATION; AVAILABILITY;
D O I
10.1371/journal.pone.0202086
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although microorganisms will preferentially allocate resources to synthesis of nitrogen (N)-acquiring enzymes when soil N availability is low according to the resource allocation model for extracellular enzyme synthesis, a robust link between microbial N-acquiring enzyme activity and soil N concentration has not been reported. To verify this link, we measured several indices of soil N availability and enzyme activity of four N-acquiring enzymes [N-acetyl-beta-glucosaminidase (NAG), protease (PR), urease (UR), and L-asparaginase (LA)] and a carbon (C)-acquiring enzyme [beta-D-glucosidase (BG)] in arable and forest soils. Although the ratios of NAG/BG and PR/BG were not significantly related with indices of soil N availability, ratios of LA/BG and UR/BG were strongly and negatively related with potentially mineralizable N estimated by aerobic incubation but not with pools of labile inorganic N and organic N. These results suggest that microorganisms might allocate their resources to LA and UR synthesis in response to N supply rate rather than the size of the easily available N pools. It was also suggested that the underlying mechanism for synthesis was different between these N-acquiring enzymes in soil microorganisms: microbial LA and UR were primarily synthesized to acquire N, whereas NAG and PR syntheses were regulated not only by N availability but also by other factors.
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页数:18
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