Effects of Pluronic F127 micelles as delivering agents on the vitro dark toxicity and photodynamic therapy activity of carboxy and pyrene substituted porphyrins

被引:20
作者
Managa, Muthumuni [1 ]
Britton, Jonathan [1 ]
Prinsloo, Earl [2 ]
Nyokong, Tebello [1 ]
机构
[1] Rhodes Univ, Dept Chem, Ctr Nanotechnol Innovat, Grahamstown, South Africa
[2] Rhodes Univ, Biotechnol Innovat Ctr, ZA-6140 Grahamstown, South Africa
基金
新加坡国家研究基金会;
关键词
Porphyrins; Photodynamic therapy; Pluronic F127 micelles; MCF-7; cells; Partition coefficient; BLOCK-COPOLYMER MICELLES; PHOTOPHYSICAL PROPERTIES; NANOPARTICLES; PHTHALOCYANINE; PHOTOSENSITIZERS; LOCALIZATION; INACTIVATION; AGGREGATION; FORMULATION; DOXORUBICIN;
D O I
10.1016/j.poly.2018.06.031
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
Metal free, Zn and ClGa containing carboxyphenoxy and phenoxy groups (complexes 1) and pyrene groups (complexes 2) were synthesized and embedded into Pluronic F127 micelles (represented as F127). Dark toxicity and photodynamic therapy activities of the embedded porphyrins were successfully studied on MCF-7 breast cancer cells. Dark toxicity showed more than 80% cell viability for all complexes. It was found that 1-Zn + F127 showed better photodynamic therapy activity compared to 1-H-2 + F127, and 1-ClGa + F127, corresponding to the high partition coefficient for the Zn porphyrin derivatives. The same applies to 2-Zn + F127 compared to 2-H-2 + F127, 2-ClGa + F127. 1-ClGa and 1-Zn were also linked to Pluronic F127 silica nanoparticles. PDT activities for embedded 1-ClGa + F127 and 1-Zn + F127 were much higher than when linked to Pluronic silica nanoparticles (PluS NPs), showing the importance of loading of porphyrins into Pluronic F127 as a drug delivering agent rather than linking. PDT studies at the highest concentration of 60 mu g/ml showed decrease in cell viability down to 15.9% for 2-Zn + F127. The K-p was determined in biphasic octanol and water system. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:102 / 107
页数:6
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