The effect of pentaghrelin on amylase release from the rat and porcine dispersed pancreatic acinar cells in vitro

被引：5
作者：
Jankowska, A.
Laubitz, D. [1 ]
Guillaume, D.
Kotunia, A.
Kapica, A.
Zabielski, R.
机构：
[1] Polish Acad Sci, Kielanowski Inst Anim Physiol & Nutr, PL-05110 Jablonna, Poland
[2] Ecole Super Agr Angers, Angers, France
[3] Agr Univ Lublin, Dept Anim Biochem & Physiol, Lublin, Poland
[4] Agr Univ Warsaw, Fac Vet Med, Dept Physiol Sci, PL-02766 Warsaw, Poland
来源：
LIVESTOCK SCIENCE | 2007年 / 108卷 / 1-3期
关键词：
pancreas; ghrelin; regulation; rat; pig;
D O I：
10.1016/j.livsci.2007.01.034
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Ghrelin-28 was found to inhibit the pancreatic enzyme output in rats, although the effect of pentaghrelin has not been studied. The effect of ghrelin on pig exocrine pancreas remains largely unknown. The aim of the present study was: (1) to establish a model of porcine dispersed pancreatic acinar cells in vitro and compare it with an existing rat model, and (2) to investigate the effect of pentaghrelin on amylase release from the rat and pig pancreatic acini. The rat and porcine acinar cell preparations released amylase in response to cholecystokinin octapeptide (CCK-8) stimulation in a dose-related manner. Pentaghrelin hardly inhibited the amylase release in rat preparations (maximum inhibition with 10(-9) M pentaghrelin). It stimulated amylase release in porcine preparations, however, no dose response was found in a range of doses between 10(-10) and 10(-7) M. Concluding, pentaghrelin may stimulate amylase release from porcine acinar cells through as yet unknown mechanisms. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：65 / 67
页数：3
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