Benzothiazole-Based Fluorescent Sensor for Ratiometric Detection of Zn(II) Ions and Secondary Sensing PPi and Its Applications for Biological Imaging and PPase Catalysis Assays

被引：76

作者：

Chang, Chengcheng ^{[1
]}

Wang, Fang ^{[1
]}

Wei, Tingwen ^{[1
]}

Chen, Xiaoqiang ^{[1
]}

机构：

[1] Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Chem Engn, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210009, Jiangsu, Peoples R China

来源：

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2017年 / 56卷 / 31期

基金：

中国国家自然科学基金;

关键词：

POLYMERASE-CHAIN-REACTION; LIVING CELLS; SELECTIVE DETECTION; AQUEOUS-SOLUTION; TURN-ON; PHYSIOLOGICAL CONDITIONS; PYROPHOSPHATE ION; ZINC IONS; ZN2+ IONS; PROBE;

D O I：

10.1021/acs.iecr.7b01209

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

In this paper, we designed and synthesized three benzothiazole-based fluorescent probes L-1, L-2, and L-3 for zinc ion detection. Among various metal ions, only the zinc ion exhibited fluorescence enhancement at 475 nm accompanied by the blue-shift emission wavelength in HEPES buffet solution containing probes. Through titration experiment, the detection limit of L-1 for zinc ion sensing was calculated to be as low as 7 nM, which showed a high sensitivity. Furthermore, the confocal laser scanning micrographs,of HeLa cells demonstrate good cell permeability of probe L-1 and selective detection of zinc ion in living cells. The L-1-Zn2+ complex was further used for pyrophosphate (PPi) sensing in HEPES buffer solution, the limit of detection was calculated to be as low as 60 nM. L-1-Zn2+ can monitor the enzyme catalyzed degradation process of PPi, thus providing a Meaningful way for tracking of zinc ion and pyrophosphate in biological systems.

引用

页码：8797 / 8805

页数：9

共 46 条

[1] Naphthalene Carbohydrazone Based Dizinc(II) Chemosensor for a Pyrophosphate Ion and Its DNA Assessment Application in Polymerase Chain Reaction Products [J].