The lysosomal membrane protein LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy

被引：10

作者：

Liu, Xuemei ^{[1
]}

Liao, Xinyi ^{[2
]}

Rao, Xiyun ^{[2
]}

Wang, Bin ^{[2
]}

Zhang, Jun ^{[2
]}

Xu, Ge ^{[2
]}

Jiang, Xuejun ^{[3
]}

Qin, Xia ^{[4
]}

Chen, Chengzhi ^{[1
,5
]}

Zou, Zhen ^{[2
,5
]}

机构：

[1] Chongqing Med Univ, Dept Occupat & Environm Hlth, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China

[3] Chongqing Med Univ, Ctr Expt Teaching Publ Hlth, Expt Teaching & Management Ctr, Chongqing, Peoples R China

[4] Chongqing Med Univ, Affiliated Hosp 1, Dept Pharm, Chongqing, Peoples R China

[5] Chongqing Med Univ, Dongsheng Lung Brain Dis Joint Lab, Chongqing, Peoples R China

来源：

FEBS LETTERS | 2020年 / 594卷 / 05期

基金：

美国国家科学基金会;

关键词：

LAMP-2; mitochondria; mitophagy; Parkin; PINK1; AUTOPHAGY; FUSION; UBIQUITIN; PARKIN; PINK1; SNARE; CARDIOMYOPATHY; DEGRADATION; PROMOTES; RECRUITS;

D O I：

10.1002/1873-3468.13663

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome-associated membrane protein 2 (LAMP-2) plays diverse functions in non-selective autophagy, chaperone-mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP-2 plays important roles during PINK1/Parkin-mediated mitophagy. The results obtained clearly show that knockdown of LAMP-2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy. The present study is the first to determine the potential role of LAMP-2 in PINK1/Parkin-mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.

引用

页码：823 / 840

页数：18

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