Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR

被引：26

作者：

Khosravi, Azar Dokht ^{[1
]}

Hoveizavi, Hajar ^{[1
]}

Mohammadian, Ali ^{[2
]}

Farahani, Abbas ^{[2
]}

Jenabi, Atefeh ^{[2
]}

机构：

[1] Ahvaz Jundishapur Univ Med Sci, Hlth Res Inst, Infect & Trop Dis Res Ctr, Ahvaz, Iran

[2] Ahvaz Jundishapur Univ Med Sci, Sch Med, Dept Microbiol, Ahvaz, Iran

来源：

ACTA CIRURGICA BRASILEIRA | 2016年 / 31卷 / 03期

关键词：

Drug Resistance; Microbial; Polymerase Chain Reaction; Genotyping Techniques; Pseudomonas aeruginosa; DIVERSITY; HOSPITALS;

D O I：

10.1590/S0102-865020160030000009

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

PURPOSE: To determine the genetic diversity of MDR P. aeruginosa strains isolated from burn and wound infections in Ahvaz, Iran, by ERIC-PCR. METHODS: From total 99 strains of P. aeruginosa defined as MDR by using drug susceptibility testing, 66 were subjected to ERIC-PCR analysis, comprises 53 strains isolated from burn infection, and 13 randomly selected strains from wound infection with higher resistance to combinations of more numbers of drugs. RESULTS: Eight clusters (I to VIII), and 50 single clones were generated for tested MDR isolates analyzed by ERIC-PCR. The high heterogeneity was observed among the isolates from burn infections including 16 isolates which were categorized in eight clusters and 37 single clones. The isolates in clusters II, III, VI, VIII showed 100% similarity. CONCLUSIONS: The high level of genotypic heterogeneity in P. aeruginosa strains demonstrated no genetic correlation between them. Extremely high drug resistance in isolates from burn, suggests that efficient control measures and proper antibiotic policy should be observed.

引用

页码：206 / 211

页数：6

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