Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

被引：12
作者：
Zhou, Wu [1 ,2 ,3 ]
Wang, Yuwei [1 ,3 ]
Zhang, Gong [1 ,3 ]
Luan, Guangxiang [1 ,3 ]
Chen, Shasha [1 ,3 ]
Meng, Jing [1 ,3 ]
Wang, Honglun [1 ,3 ]
Hu, Na [1 ]
Suo, Yourui [1 ,2 ]
机构：
[1] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810008, Qinghai, Peoples R China
[2] Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
MOLECULES | 2018年 / 23卷 / 05期
关键词：
Hippophae rhamnoides L; dioecious; sex determination; RAPD; SCAR; CHROMOSOME EVOLUTION; FLOWERING PLANTS;
D O I：
10.3390/molecules23051048
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5-CATCCGTGCT-3) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.
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页数：9
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