Parkin and synphilin-1 isoform expression changes in Lewy body diseases

被引：34
作者：
Humbert, Jordi
Beyer, Katrin [1 ]
Carrato, Cristina
Mate, Jose L.
Ferrer, Isidro
Ariza, Aurelio
机构：
[1] Univ Autonoma Barcelona, Hosp Germans Trias & Pujol, Dept Pathol, Badalona 08916, Spain
[2] Hosp Bellvitge Princeps Espanya, Inst Neuropathol, Lhospitalet De Llobregat, Spain
来源：
NEUROBIOLOGY OF DISEASE | 2007年 / 26卷 / 03期
关键词：
dementia with lewy bodies; Parkinson disease; lewy body variant of Alzheimer disease; differential isoform expression; transcript variant;
D O I：
10.1016/j.nbd.2007.03.007
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Alternative splicing gives rise to at least seven parkin and eight synphilin-1 isoforms. Since both parkin and synphifn-1 have been involved in Lewy body (LB) formation, we decided to explore whether their isoforms are differentially expressed in LB diseases. With this aim, we studied relative mRNA expression levels of parkin and synphilin-1 isoforms in the frontal cortices of patients with dementia with LBs, the LB variant of Alzheimer's disease and Parkinson's disease and compared the findings with those obtained from Alzheimer's disease patients and control individuals. Duplex real-time PCR reactions, with beta-actin as internal standard, were carried out in a LightCycler. mRNA expression levels of parkin and synphilin-1 isoforms were seen to be specifically altered in each of the LB diseases studied. These findings suggest that parkin and synphilin-1 isoform expression changes play a significant role in the pathogenesis of LB diseases. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：681 / 687
页数：7
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