Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging

被引：9

作者：

Tripathi, Madhulika ^{[1
]}

Yen, Paul Michael ^{[1
,2
]}

Singh, Brijesh Kumar ^{[1
]}

机构：

[1] Duke NUS Med Sch, Cardiovasc & Metab Disorders Program, Singapore 169857, Singapore

[2] Duke Univ, Sch Med, Durham, NC USA

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 02期

基金：

英国医学研究理事会;

关键词：

MARKER;

D O I：

10.1016/j.xpro.2020.100064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously developed senescent primary fibroblast models have limited rele-vance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These se-nescent AML12 cells should be a useful in vitro model to study the metabolic ef-fects of aging in the liver. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).

引用

页数：10

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