Focal modulation microscopy with annular apertures: A numerical study

被引：22

作者：

Gong, Wei ^{[1
]}

Si, Ke ^{[2
]}

Chen, Nanguang ^{[1
,3
]}

Sheppard, Cohn J. R. ^{[1
,2
,4
]}

机构：

[1] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore 117456, Singapore

[3] Natl Univ Singapore, Dept Elect & Comp Engn, Singapore 117576, Singapore

[4] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 07期

关键词：

Confocal microscopy; annular apertures; phase modulation; spatial resolution; penetration depth; background rejection; LINE-SCANNING MICROSCOPE; RESOLUTION;

D O I：

10.1002/jbio.200900110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Image formation of focal modulation microscopy with annular aperture (AFMM) is presented. The spatial resolution is discussed. Compared with confocal microscopy, AFMM can simultaneously enhance the axial and transverse resolution. By adjusting the width of the annular objective aperture, AFMM can be adjusted from best spatial resolution performance to highest signal level. The capability of background rejection is also investigated, showing AFMM has the potential to further increase the imaging penetration depth. Finally, an optimal configuration of AFMM is elaborated. [GRAPHICS] Radial spoke pattern at the focal plane for focal modulation microscopy with annular apertures (AFMM).

引用

页码：476 / 484

页数：9

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