Isolation and partial characterization of cDNA clone of human ceruloplasmin receptor

被引:0
作者
Sasina, LK [1 ]
Tsymbalenko, NV [1 ]
Platonova, NA [1 ]
Puchkova, LV [1 ]
Voronina, OV [1 ]
Gyulikhandanova, NE [1 ]
Gaitskhoki, VS [1 ]
机构
[1] Russian Acad Med Sci, Inst Expt Med, Dept Mol Genet, St Petersburg, Russia
基金
俄罗斯基础研究基金会;
关键词
ceruloplasmin receptor; clotting; cDNA sequencing; copper metabolism;
D O I
10.1007/BF02439812
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
An individual clone, presumably carrying a 3 bp fragment of ceruloplasmin receptor cDNA was isolated from the expression library of human placenta cDNA using polyclonal specific antibodies to ceruloplasmin receptors. EcoR1-hydrolysate of isolated DNA was cloned in a pTZ19 bacterial vector and sequenced in the forward and reverse direction. The comparison of the revealed sequence with known sequences of human genome revealed its high similarity to ceruloplasmin cDNA.
引用
收藏
页码:491 / 495
页数:5
相关论文
共 10 条
[1]   THE FET3 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A MULTICOPPER OXIDASE REQUIRED FOR FERROUS IRON UPTAKE [J].
ASKWITH, C ;
EIDE, D ;
VANHO, A ;
BERNARD, PS ;
LI, LT ;
DAVISKAPLAN, S ;
SIPE, DM ;
KAPLAN, J .
CELL, 1994, 76 (02) :403-410
[2]   CERULOPLASMIN RECEPTORS OF ERYTHROCYTES [J].
BARNES, G ;
FRIEDEN, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 125 (01) :157-162
[3]   FINE-STRUCTURE OF THE HUMAN CERULOPLASMIN GENE [J].
DAIMON, M ;
YAMATANI, K ;
IGARASHI, M ;
FUKASE, N ;
KAWANAMI, T ;
KATO, T ;
TOMINAGA, M ;
SASAKI, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 208 (03) :1028-1035
[4]  
HARRIS ED, 1991, SOC EXP BIOL MED, V28, P130
[5]  
PATEL BN, 1997, J BIOL CHEM, V272, P20285
[6]  
Puchkova L V, 1990, Biokhimiia, V55, P2182
[7]  
PUCHKOVA LV, 1991, BIOKHIMIYA, V56, P3261
[8]  
SAMBROOK JI, 1991, MOL CLONING
[9]   Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse [J].
Vulpe, CD ;
Kuo, YM ;
Murphy, TL ;
Cowley, L ;
Askwith, C ;
Libina, N ;
Gitschier, J ;
Anderson, GJ .
NATURE GENETICS, 1999, 21 (02) :195-199
[10]   The X-ray structure of human serum ceruloplasmin at 3.1 angstrom: Nature of the copper centres [J].
Zaitseva, I ;
Zaitsev, V ;
Card, G ;
Moshkov, K ;
Bax, B ;
Ralph, A ;
Lindley, P .
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 1996, 1 (01) :15-23