Identification and Characterization of the HicAB Toxin-Antitoxin System in the Opportunistic Pathogen Pseudomonas aeruginosa

被引:45
作者
Li, Gang [1 ]
Shen, Mengyu [1 ]
Lu, Shuguang [1 ]
Le, Shuai [1 ]
Tan, Yinling [1 ]
Wang, Jing [1 ]
Zhao, Xia [1 ]
Shen, Wei [1 ]
Guo, Keke [1 ]
Yang, Yuhui [1 ]
Zhu, Hongbin [1 ]
Rao, Xiancai [1 ]
Hu, Fuquan [1 ]
Li, Ming [1 ]
机构
[1] Third Mil Med Univ, Dept Microbiol, Chongqing 400038, Peoples R China
来源
TOXINS | 2016年 / 8卷 / 04期
基金
中国国家自然科学基金;
关键词
toxin-antitoxin system; HicAB; Pseudomonas aeruginosa; biofilm formation; virulence; PERSISTER CELL-FORMATION; ESCHERICHIA-COLI; BURKHOLDERIA-PSEUDOMALLEI; BIOFILM FORMATION; YERSINIA-PESTIS; MESSENGER-RNA; F-PLASMID; BACTERIA; ARCHAEA; AGGREGATION;
D O I
10.3390/toxins8040113
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Toxin-antitoxin (TA) systems are small genetic modules that are widely distributed in the genomes of bacteria and archaea and have been proposed to fulfill numerous functions. Here, we describe the identification and characterization of a type II TA system, comprising the hicAB locus in the human opportunistic pathogen Pseudomonas aeruginosa. The hicAB locus consists of genes hicA and hicB encoding a toxin and its cognate antitoxin, respectively. BLAST analysis revealed that hicAB is prevalent in approximately 36% of P. aeruginosa strains and locates in the same genomic region. RT-PCR demonstrated that hicAB forms a bicistronic operon that is cotranscribed under normal growth conditions. Overproduction of HicA inhibited the growth of Escherichia coli, and this effect could be counteracted by co-expression of HicB. The Escherichia coli kill/rescue assay showed that the effect of HicA is bacteriostatic, rather than bactericidal. Deletion of hicAB had no effect on the biofilm formation and virulence of P. aeruginosa in a mice infection model. Collectively, this study presents the first characterization of the HicAB system in the opportunistic pathogen P. aeruginosa.
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页数:12
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共 48 条
[1]   Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis [J].
Agarwal, Shivangi ;
Agarwal, Shivani ;
Bhatnagar, Rakesh .
FEBS LETTERS, 2007, 581 (09) :1727-1734
[2]   Functional and Structural Analysis of HicA3-HicB3, a Novel Toxin-Antitoxin System of Yersinia pestis [J].
Bibi-Triki, Sabrina ;
Li de la Sierra-Gallay, Ines ;
Lazar, Noureddine ;
Leroy, Arnaud ;
Van Tilbeurgh, Herman ;
Sebbane, Florent ;
Pradel, Elizabeth .
JOURNAL OF BACTERIOLOGY, 2014, 196 (21) :3712-3723
[3]   The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation [J].
Butt, Aaron ;
Higman, Victoria A. ;
Williams, Christopher ;
Crump, Matthew P. ;
Hemsley, Claudia M. ;
Harmer, Nicholas ;
Titball, Richard W. .
BIOCHEMICAL JOURNAL, 2014, 459 :333-344
[4]   Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei [J].
Butt, Aaron ;
Mueller, Claudia ;
Harmer, Nicholas ;
Titball, Richard W. .
FEMS MICROBIOLOGY LETTERS, 2013, 338 (01) :86-94
[5]   Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress [J].
Cheng, Hsin-Yao ;
Soo, Valerie W. C. ;
Islam, Sabina ;
McAnulty, Michael J. ;
Benedik, Michael J. ;
Wood, Thomas K. .
ENVIRONMENTAL MICROBIOLOGY, 2014, 16 (06) :1741-1754
[6]   A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells:: Application for DNA fragment transfer between chromosomes and plasmid transformation [J].
Choi, KH ;
Kumar, A ;
Schweizer, HP .
JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 64 (03) :391-397
[7]   Activation of Toxin-Antitoxin System Toxins Suppresses Lethality Caused by the Loss of σE in Escherichia coli [J].
Daimon, Yasushi ;
Narita, Shin-ichiro ;
Akiyama, Yoshinori .
JOURNAL OF BACTERIOLOGY, 2015, 197 (14) :2316-2324
[8]   Crystal structure of uncharacterized protein TTHA1756 from Thermus thermaphilus HB8:: Structural variety in UPF0150 family proteins [J].
Ebihara, Akio ;
Manzoku, Miho ;
Iino, Hitoshi ;
Kanagawa, Mayumi ;
Shinkai, Akeo ;
Yokoyama, Shigeyuki ;
Kuramitsu, Seiki .
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2008, 71 (04) :2097-2101
[9]   Toxin-Antitoxin Systems as Multilevel Interaction Systems [J].
Goeders, Nathalie ;
Van Melderen, Laurence .
TOXINS, 2014, 6 (01) :304-324
[10]   The Yersinia pestis Chromosome Encodes Active Addiction Toxins [J].
Goulard, Celine ;
Langrand, Sophie ;
Carniel, Elisabeth ;
Chauvaux, Sylvie .
JOURNAL OF BACTERIOLOGY, 2010, 192 (14) :3669-3677