Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway

被引：43
作者：
Chen, Lei [1 ]
Li, Kang [1 ]
Liu, Qian [1 ]
Quiles, Jose L. [2 ]
Filosa, Rosanna [3 ]
Kamal, Mohammad Amjad [4 ,5 ,6 ]
Wang, Fang [7 ]
Kai, Guoyin [8 ]
Zou, Xiaobo [9 ]
Teng, Hui [1 ]
Xiao, Jianbo [9 ]
机构：
[1] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China
[2] Univ Granada, Inst Nutr & Food Technol Jose Mataix, Dept Physiol, Biomed Res Ctr, Granada 18100, Spain
[3] Univ Campania, Dept Expt Med, Via L De Crecchio 7, I-80138 Naples, Italy
[4] King Abdulaziz Univ, King Fahd Med Res Ctr, POB 80216, Jeddah 21589, Saudi Arabia
[5] Enzymoics, 7 Peterlee Pl, Hebersham, NSW 2770, Australia
[6] Novel Global Community Educ Fdn, Hebersham, Australia
[7] Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China
[8] Zhejiang Chinese Med Univ, Coll Pharm, Lab Med Plant Biotechnol, Hangzhou 311402, Zhejiang, Peoples R China
[9] Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Jiangsu, Peoples R China
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2019年 / 133卷
基金：
中国国家自然科学基金;
关键词：
Raspberry; Oxidative damage; HepG2; cells; Keap1/Nrf2; pathway; Cyanidin; 3-O-Glucoside; MOLECULAR-MECHANISMS; RED RASPBERRY; STRESS; NRF2; ANTHOCYANINS; DNA; POLYPHENOLS; ACTIVATION; EXPRESSION; CAPACITY;
D O I：
10.1016/j.fct.2019.110781
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against H2O2-induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by H2O2 and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and gamma-GCS. These results taken together indicated that raspberry treatment could ameliorate H2O2-induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.
引用
收藏
页数：8
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