Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

被引:23
作者
Kim, Min Kyu
Hossein, Mohammad Shamim
Oh, Hyun Ju
Fibrianto, Heru Yuda
Jang, Goo
Kim, Hye Jin
Hong, So Gun
Park, Jung Eun
Kang, Sung Keun
Lee, Byeong Chun
机构
[1] Seoul Natl Univ, Coll Vet Med, Dept Theriogenol & Biotechnol, Seoul 151742, South Korea
[2] Haemaru Small Anim Referral Hosp, Kyonggi Do 463050, South Korea
关键词
canine oocyte; GSH; in vitro; in vivo; reproductive stage;
D O I
10.1292/jvms.69.627
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 mu m and > 120 mu m in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 mu g/ml estrogen, 0.5 mu g/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 mu M beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 mu m and > 120 mu m in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P < 0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P < 0.05). Presence of P-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus Oocytes.
引用
收藏
页码:627 / 632
页数:6
相关论文
共 46 条
[1]   In vitro maturation and glutathione synthesis of porcine oocytes in the presence or absence of cysteamine under different oxygen tensions:: role of cumulus cells [J].
Bing, YZ ;
Hirao, Y ;
Iga, K ;
Che, LM ;
Takenouchi, N ;
Kuwayama, M ;
Fuchimoto, D ;
Rodriguez-Martinez, H ;
Nagai, T .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2002, 14 (03) :125-131
[2]   OOCYTE AND FOLLICULAR MORPHOLOGY AS DETERMINING CHARACTERISTICS FOR DEVELOPMENTAL COMPETENCE IN BOVINE OOCYTES [J].
BLONDIN, P ;
SIRARD, MA .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1995, 41 (01) :54-62
[3]   In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential [J].
Bolamba, D ;
Russ, KD ;
Olson, MA ;
Sandler, JL ;
Durrant, BS .
THERIOGENOLOGY, 2002, 58 (09) :1689-1703
[4]   Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes [J].
Brad, AM ;
Bormann, CL ;
Swain, JE ;
Durkin, RE ;
Johnson, AE ;
Clifford, AL ;
Krisher, RL .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2003, 64 (04) :492-498
[5]  
CINONE M, 1992, P 12 INT C AN REPR H, V4, P1767
[6]   Capacity of adult and prepubertal mouse oocytes to undergo embryo development in the presence of cysteamine [J].
de Matos, DG ;
Nogueira, D ;
Cortvrindt, R ;
Herrera, C ;
Adriaenssens, T ;
Pasqualini, RS ;
Smitz, J .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2003, 64 (02) :214-218
[7]   Cysteamine supplementation during in vitro maturation and embryo culture: A useful tool for increasing the efficiency of bovine in vitro embryo production [J].
de Matos, DG ;
Herrera, C ;
Cortvrindt, R ;
Smitz, J ;
Van Soom, A ;
Nogueira, D ;
Pasqualini, RS .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2002, 62 (02) :203-209
[8]  
deMatos DG, 1996, MOL REPROD DEV, V45, P451, DOI 10.1002/(SICI)1098-2795(199612)45:4&lt
[9]  
451::AID-MRD7&gt
[10]  
3.0.CO