Protein-fragment complementation assays for large-scale analysis of protein-protein interactions

被引：19

作者：

Blaszczak, Ewa ^{[1
]}

Lazarewicz, Natalia ^{[1
,2
]}

Sudevan, Aswani ^{[2
]}

Wysocki, Robert ^{[1
]}

Rabut, Gwenael ^{[2
]}

机构：

[1] Univ Wroclaw, Fac Biol Sci, Dept Genet & Cell Physiol, Kanonia 6-8, PL-50328 Wroclaw, Poland

[2] Univ Rennes, IGDR Inst Genet & Dev Rennes, CNRS, UMR 6290, F-35000 Rennes, France

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2021年 / 49卷 / 03期

关键词：

BIMOLECULAR FLUORESCENCE COMPLEMENTATION; IN-VIVO; DIHYDROFOLATE-REDUCTASE; DRUG DISCOVERY; LIVING CELLS; CDNA LIBRARY; MAP; REPORTER; VISUALIZATION; COMPLEXES;

D O I：

10.1042/BST20201058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-protein interactions (PPIs) orchestrate nearly all biological processes. They are also considered attractive drug targets for treating many human diseases, including cancers and neurodegenerative disorders. Protein-fragment complementation assays (PCAs) provide a direct and straightforward way to study PPIs in living cells or multicellular organisms. Importantly, PCAs can be used to detect the interaction of proteins expressed at endogenous levels in their native cellular environment. In this review, we present the principle of PCAs and discuss some of their advantages and limitations. We describe their application in large-scale experiments to investigate PPI networks and to screen or profile PPI targeting compounds.

引用

页码：1337 / 1348

页数：12

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