Phospholipase C is required for the control of stomatal aperture by ABA

被引：104
作者：
Hunt, L
Mills, LN
Pical, C
Leckie, CP
Aitken, FL
Kopka, J
Mueller-Roeber, B
McAinsh, MR
Hetherington, AM
Gray, JE
机构：
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
[2] Univ Lancaster, Div Biol Sci, Lancaster LA1 4YQ, England
[3] Lund Univ, Dept Plant Biochem, Ctr Chem & Chem Engn, SE-22100 Lund, Sweden
[4] Newcastle Univ, Sch Med, Dept Physiol Sci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[5] Max Planck Inst Mol Pflanzenphysiol, D-14424 Potsdam, Germany
[6] Univ Potsdam, Mol Biol Abt, Inst Biochem & Biol, D-14476 Golm, Germany
来源：
PLANT JOURNAL | 2003年 / 34卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
guard cell; calcium; abscisic acid; inositol 1,4,5-trisphosphate;
D O I：
10.1046/j.1365-313X.2003.01698.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.
引用
收藏
页码：47 / 55
页数：9
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