Human CD4+CD25+ regulatory T cells inhibit the differentiation of osteoclasts from peripheral blood mononuclear cells

被引：150
作者：
Kim, Yong Gil
Lee, Chang-Keun
Nah, Seong-Su
Mun, Se Hwan
Yoo, Bin
Moon, Hee-Bom
机构：
[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Internal Med,Div Theumatol, Seoul 138736, South Korea
[2] Asan Inst Life Sci, Seoul, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 04期
关键词：
regulatory T-lymphocyte; osteoclast; TGF-beta; IL-4;
D O I：
10.1016/j.bbrc.2007.04.042
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Regulatory T cell (Treg) is a subset of CD4+ T lymphocytes expressing CD25 with immunosuppressive activity. However the function of Tregs onto osteoclastogenesis remains unknown. We investigated the effect and regulatory mechanism of Treg focusing on osteoclastogenesis from PBMCs. Tregs were isolated from PBMCs by magnetic cell sorting-column and analyzed by flow cytometry. RT-PCR was performed to identify Foxp3 mRNA. Using PBMCs and Tregs coculture system, we could find that Tregs inhibited osteoclasts differentiation from PBMCs and reduced the resorbed areas on pit assay (p < 0.01). This suppression of osteoclast differentiation was cytokine-dependent, not cell-to-cell direct contact proved by Transwell system. Tregs-induced osteoclast differentiation was blocked by anti-TGF-beta or anti-IL-4 antibody treatment. These results suggest that Tregs inhibit osteoclast differentiation from PBMCs in a cytokine-dependent manner, not by cell-to-cell contact manner and that TGF-beta and IL-4 may be the key cytokines for this suppressive function of Tregs. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：1046 / 1052
页数：7
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